This Sox10ERT2CreERT2 (SECE17) strain, carrying a transgene with tamoxifen inducible Cre recombinase under the control of the Sox10 MCS4 enhancer/c-Fos minimal promoter, may be useful for generating spatiotemporal neural crest cell specific targeted mutants for studies of neural crest-derived cell populations during embryonic development.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
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|
Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
These transgenic mice express a tamoxifen inducible Cre recombinase, ERT2/cre/ERT2, driven by the mouse Sox10 MCS4 enhancer/c-Fos minimal promoter.
ERT2/cre/ERT2 (also known as ERiCreER) has been shown to tightly regulate inducible Cre recombinase activity with no detectable recombination activity without 4-hydroxytamoxifen.
When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in neural crest cells. Heterozygotes are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous, but predicts homozygotes would be viable.
The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 and ERT2/cre/ERT2 (also known as ERiCreER) can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.
A transgenic insert designed by Dr. Philippe Soriano (School of Medicine at Mount Sinai)
containing Cre recombinase coding sequences flanked by two ERT2 domains (from the pCAGERT2CreERT2 plasmid), followed by the rabbit globin poly-adenylation sequence,
under the control of the mouse Sox10 MCS4 enhancer/c-Fos minimal promoter, was injected into C57BL/6 X C3H zygotes. Founder line SECE17 was subsequently established. The mice were then backcrossed to 129S4/SvJaeSor for 9 generations.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize 129S4/SvJaeJ
oocytes (Stock No. 009104).
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | Tamoxifen administration induces Cre recombination in neural crest cells. |
Allele Name | transgene insertion 17, Philippe Soriano |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | SECE17; Sox10ERT2CreERT2 |
Gene Symbol and Name | Tg(Sox10-ERT2/cre/ERT2)17Sor, transgene insertion 17, Philippe Soriano |
Gene Synonym(s) | |
Promoter | Sox10, SRY (sex determining region Y)-box 10, mouse, laboratory |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | Tamoxifen administration induces Cre recombination in neural crest cells. |
Strain of Origin | C57BL/6 x C3H |
Chromosome | UN |
Molecular Note | The Multiple Species Conserved Sequence 4 (MCS 4) distal enhancer with a c-Fos minimal promoter drives neural crest cell and derivatives-specific expression of an inducible cre recombinase gene flanked by two modified estrogen receptors (ERT2/cre/ERT2) followed by a rabbit globin polyadenylation sequence. Two lines were generated with line 17 selected as the representative line. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to 129S4/SvJaeJ inbred mice (Stock No. 009104). The Donating Investigator has not attempted to make the strain homozygous, but predicts homozygotes would be viable.
When using the SECE17 mouse strain in a publication, please cite the originating article(s) and include JAX stock #028658 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Sox10-ERT2/cre/ERT2)17Sor |
Frozen Mouse Embryo | 129S4.Cg-Tg(Sox10-ERT2/cre/ERT2)17Sor/J | $2595.00 |
Frozen Mouse Embryo | 129S4.Cg-Tg(Sox10-ERT2/cre/ERT2)17Sor/J | $2595.00 |
Frozen Mouse Embryo | 129S4.Cg-Tg(Sox10-ERT2/cre/ERT2)17Sor/J | $3373.50 |
Frozen Mouse Embryo | 129S4.Cg-Tg(Sox10-ERT2/cre/ERT2)17Sor/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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