This Prop1 knockout allele is useful in studies of Wnt/β-catenin signaling during processes of cell-lineage determination, development and disease.
Michael Geoff Rosenfeld, University of California, San Diego
PROP1 (paired like homeodomain factor 1) protein has been found to complex with CTNNB1 (catenin (cadherin associated protein), beta 1; also called β-catenin) to influence cell-lineage determination in processes of development and disease.
All but the first 8 amino acids of the mouse Prop1 gene were replaced by a lacZ-neomycin resistance cassette to create this null allele. Absence of protein has been demonstrated by immunostaining of the pituitary gland. Mice homozygous for the knockout are dwarf, and exhibit reduced body size and weight. Homozygous knockout mice fully recapitulate the essential features of Prop1df (Ames dwarf) allele, including loss of Pit1-dependent pituitary cell types and dysmorphogenesis of the anterior lobe. Homozygotes are infertile.
The entire locus beyond the first 8 amino acids of coding sequence was replaced by a lacZ-neomycin cassette by homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for more than 10 generations (see SNP note below).
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 43 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Michael G Rosenfeld|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Prop1, paired like homeodomain factor 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The entire locus beyond the first 8 amino acids of coding sequence was replaced by a lacZ-neo. Absence of protein was demonstrated by immunostaining.|
Heterozygotes are viable and fertile. Homozygotes are infertile.
When using the Prop1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #028632 in your Materials and Methods section.