B6.129-Prop1^tm1Rsd/J

Stock number: 028632
Product name: Prop1^-
Research areas: Developmental Biology Research, Endocrine Deficiency Research, Reproductive Biology Research

Description

This Prop1 knockout allele is useful in studies of Wnt/β-catenin signaling during processes of cell-lineage determination, development and disease.

Detailed description

PROP1 (paired like homeodomain factor 1) protein has been found to complex with CTNNB1 (catenin (cadherin associated protein), beta 1; also called β-catenin) to influence cell-lineage determination in processes of development and disease.

All but the first 8 amino acids of the mouse Prop1 gene were replaced by a lacZ-neomycin resistance cassette to create this null allele. Absence of protein has been demonstrated by immunostaining of the pituitary gland. Mice homozygous for the knockout are dwarf, and exhibit reduced body size and weight. Homozygous knockout mice fully recapitulate the essential features of Prop1^df (Ames dwarf) allele, including loss of Pit1-dependent pituitary cell types and dysmorphogenesis of the anterior lobe. Homozygotes are infertile.

Development

The entire locus beyond the first 8 amino acids of coding sequence was replaced by a lacZ-neomycin cassette by homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for more than 10 generations (see SNP note below).

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 43 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Prop1^tm1Rsd
Name: targeted mutation 1, Michael G Rosenfeld
MGI accession ID: MGI:3690463
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Prop1^-
Marker symbol: Prop1
Marker name: paired like homeodomain factor 1
Marker synonyms: CPHD2; Prop-1; prophet of Pit1; prophet of Pit-1
Chromosome: 11
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: The entire locus beyond the first 8 amino acids of coding sequence was replaced by a lacZ-neo. Absence of protein was demonstrated by immunostaining.