B6.Cg-Ccn3^tm1.1Jt/J

Stock number: 028614
Product name: Nov^flx
Research areas: Neurobiology Research, Research Tools

Description

Exons 3 and 4 of the mouse Nov gene are flanked by loxP sites. Brain-specific cre-mediated excision of the floxed region in the brain results in a mild anxiolytic effect.

Detailed description

Exons 3 and 4 of mouse Nov (nephroblastoma overexpressed gene) is flanked by loxP sites. Cre-mediated excision of the floxed region results in a knockout allele.

Crosses with CamKIIA-Cre mice result in a knockout of Nov expression in brain, and a mild anxiolytic (anxiety-inhibiting) effect is observed. There is no obvious effect on the circadian clock.

Development

BAC clone RP24-289J3, containing the mouse Nov gene was used to introduce an FRT-flanked neomycin resistance gene followed by a loxP site in intron 2, and another loxP site in intron 4. The mutation was created through homologous recombination in B6(Cg)-Tyr^c-2J/J-derived LR2-6-1 embryonic stem (ES) cells. Resultant chimeric mice were crossed to B6;SJL-Tg(ACTFLPe)9205Dym/J animals (see Stock No. 003800) to excise the neomycin cassette. This strain has been backcrossed to C57BL/6J for 6 generations by the donating lab.

Allele

Symbol: Ccn3^tm1.1Jt
Name: targeted mutation 1.1, Joseph S Takahashi
MGI accession ID: MGI:5751910
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ccn3
Marker name: cellular communication network factor 3
Chromosome: 15
Strain of origin: B6(Cg)-Tyr^c-2J/J
Molecular note: BAC clone RP24-289J3, containing the mouse Nov gene, was used to introduce an FRT-flanked neomycin resistance gene followed by a loxP site in intron 2, and another loxP site in intron 4. Flp-mediated recombination removed the FRT-flanked neo cassette.
General note: ES cells = LR2-6-1