Exons 3 and 4 of the mouse Nov gene are flanked by loxP sites. Brain-specific cre-mediated excision of the floxed region in the brain results in a mild anxiolytic effect.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Exons 3 and 4 of mouse Nov (nephroblastoma overexpressed gene) is flanked by loxP sites. Cre-mediated excision of the floxed region results in a knockout allele.
Crosses with CamKIIA-Cre mice result in a knockout of Nov expression in brain, and a mild anxiolytic (anxiety-inhibiting) effect is observed. There is no obvious effect on the circadian clock.
BAC clone RP24-289J3, containing the mouse Nov gene was used to introduce an FRT-flanked neomycin resistance gene followed by a loxP site in intron 2, and another loxP site in intron 4. The mutation was created through homologous recombination in B6(Cg)-Tyrc-2J/J-derived LR2-6-1 embryonic stem (ES) cells. Resultant chimeric mice were crossed to B6;SJL-Tg(ACTFLPe)9205Dym/J animals (see Stock No. 003800) to excise the neomycin cassette. This strain has been backcrossed to C57BL/6J for 6 generations by the donating lab.
|Allele Name||targeted mutation 1.1, Joseph S Takahashi|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ccn3, cellular communication network factor 3|
|Strain of Origin||B6(Cg)-Tyrc-2J/J|
|General Note||ES cells = LR2-6-1|
|Molecular Note||BAC clone RP24-289J3, containing the mouse Nov gene, was used to introduce an FRT-flanked neomycin resistance gene followed by a loxP site in intron 2, and another loxP site in intron 4. Flp-mediated recombination removed the FRT-flanked neo cassette.|
Homozygous and heterozygous floxed mice are viable and fertile.
When using the Novflx mouse strain in a publication, please cite the originating article(s) and include JAX stock #028614 in your Materials and Methods section.