Cnr1-eGFP-miGAD67 line 1 BAC transgenic mice have expression of both the eGFP fluorescent protein and a synthetic miRNA sequence (miRNA:Gad1 or miGAD67) that targets the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA for knockdown. Expression is directed by the mouse cannabinoid receptor 1 promoter/enhancer regions on the BAC. 
The morphology and distribution of the labeled interneurons across the different brain structures show that the transgene is specifically expressed in the cell sub-populations that normally express the Cnr1 gene; including GABAergic interneuron basket cells (smaller subpopulation of CCK+ basket cells). 
CNR1+ cells exhibit non-detectable levels of GAD1 expression; resulting in complex behavioral and molecular disturbances, altered responses to cannabinoid and psychostimulant drugs, and increased preference for social novelty.
Strong eGFP fluorescence is reported specifically in CNR1+ cells across all tested brain regions (cortex, hippocampus, substantia nigra, amygdala and cerebellum), with strong labeling of neuronal somata and synaptic terminals.
 Hemizygous mice are viable and fertile. To date (December 2015), it has not been attempted to make this strain homozygous.
 The miRNA:Gad1-containing &beta;-globin minigene is flanked with lox2272 sites, which allows excision of the GAD1 gene-silencing aspect of the transgene while retaining the eGFP fluorescence in CNR1+ cells.

Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Cnr1-eGFP-miGAD67 line 1 BAC transgenic mouse line is a hybrid reporter/miRNA knockdown model where cannabinoid receptor 1 interneuronal expression of a synthetic Gad1 miRNA precursor results in Gad1 transcript downregulation (silencing of GAD1 expression) via endogenous miRNA processing mechanisms. These mice may be useful in studying CNR1+ GABAergic interneurons in behavioral and molecular processes, as well as cell-type specific GABAergic disturbances in manifestations of schizophrenia.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

<a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> for more information.