Npy-eGFP-miGAD67 line 1 BAC transgenic mice have expression of both the eGFP fluorescent protein and a synthetic miRNA sequence (miRNA:Gad1 or miGAD67) that targets the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA for knockdown. Expression is directed by the mouse neuropeptide Y promoter/enhancer regions on the BAC. 
The morphology and distribution of the labeled interneurons across the different brain structures show that the transgene is specifically expressed in the cell sub-populations that normally express the Npy gene; including interneuron neurogliaform cells (primarily signal through nonsynaptic volume transmission) and Martinotti cells (synapse onto dendrites in amygdala, cortex, hippocampus, hypothalamus and striatum). 
NPY+ cells exhibit non-detectable levels of GAD1 expression; resulting in reduced anxiety, increased preference for social novelty, increased PEP19/PCP4, increased amphetamine sensitivity.
Strong eGFP fluorescence is reported specifically in NPY+ cells. 
 The donating investigator reports that hemizygous and homozygous mice are viable and fertile. 
 The miRNA:Gad1-containing &beta;-globin minigene is flanked with lox2272 sites, which allows excision of the GAD1 gene-silencing aspect of the transgene while retaining the eGFP fluorescence in NPY+ cells.

The Npy-eGFP-miGAD67 line 1 BAC transgenic mouse line is a hybrid reporter/miRNA knockdown model where neuropeptide Y interneuronal expression of a synthetic Gad1 miRNA precursor results in Gad1 transcript downregulation (silencing of GAD1 expression) via endogenous miRNA processing mechanisms. These mice may be useful in studying NPY+ GABAergic interneurons in behavioral and molecular processes, as well as cell-type specific GABAergic disturbances in manifestations of schizophrenia.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

Typically mice are recovered in 12-16 weeks. <a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> to place an order or for more information.