Of note, the same Sst-ires-Flp allele will also be available on a C57BL/6J genetic background as Stock No. 031629.
Sst-ires-Flp knock-in mice (or SOM-IRES-Cre) have FLPo recombinase expression in GABAergic neurons derived from the medial-ganglionic eminence (MGE) - as directed by the endogenous somatostatin promoter. These mice are useful for studying the fate of progenitor cells at the SST/CR intersection.Z. Josh Huang, Cold Spring Harbor Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing) | Sst | somatostatin |
Sst-ires-Flp knock-in mice express Flp Recombinase from the endogenous somatostatin (Sst) promoter/enhancer elements. SST expressing cells constitute half of the GABAergic neurons along the medial-ganglionic eminence and the preoptic areas (MGE-POA) and up to 30% all GABA neurons in many cortical areas. Homozygous mice are viable and fertile. FLP is detected in somatostatin positive neurons across layer 2 to 6, including dendritic inhibitory interneurons such as Martinotti cells and Oriens-Lacunosum-Moleculare (O-LM) cells. As such, when Sst-ires-Flp mice are bred with mice containing frt-flanked sequence(s), Flp-mediated recombination will result in deletion of the flanked sequence(s) in the Sst-expressing cells of the offspring.
These mice were bred to Ai65(RCFL-tdT)-neo mice (Stock No. 021875) (after removal of the loxP-flanked STOP codon by breeding with CR(calretinin)-ires-cre mice (Stock No. 010774) in order to study the fate of progenitor cells at the SST/CR intersection. tdTomato-labeled cells are enriched in layers 2 and 3 (L2/3), L5a, and L6 but are almost absent in L4. The L2/3 SST/CR cells have Martinotti cell morphology with an ascending axon that arborizes in L1 with fewer branches in L2/3. The L5 SST/CR neurons also have Martinotti cell morphology with ascending axons that invade layer 1. These neurons have adapting firing patterns.
The Sst-ires-Flp knock-in allele (or SOM-IRES-Cre) was designed by Dr. Z. Josh Huang (Cold Spring Harbor Laboratory).
A targeting vector was designed to insert an internal ribosome entry site (IRES) fused to the FLPo recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), followed by a loxP-flanked neo cassette, downstream of the stop codon of the somatostatin locus (Sst) on chromosome 16. This construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric animals were bred with B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054) to generate the colony and remove the neo selection cassette. The resulting Sst-ires-Flp mice were bred to C57BL/6J mice to remove the Cre-expressing transgene, and then offspring were subsequently bred together to create a homozygous Sst-ires-Flp colony.
Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | FLP, FLP recombinase, Saccharomyces cerevisiae |
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Site of Expression | FLP Recombinase is detected in somatostatin positive neurons. |
Allele Name | targeted mutation 3.1, Z Josh Huang |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | somatostatin-Flp; Som-Flp; SOM-IRES-Flp; SST-Flp; SST-ires-Flpo |
Gene Symbol and Name | Sst, somatostatin |
Gene Synonym(s) | |
Expressed Gene | FLP, FLP recombinase, Saccharomyces cerevisiae |
Site of Expression | FLP Recombinase is detected in somatostatin positive neurons. |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 16 |
Molecular Note | The targeting vector is designed to insert an internal ribosome entry site (IRES) fused to flpo recombinase gene (a codon-optimized flpe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), followed by a loxP-flanked neo cassette, downstream of the stop codon of the gene. Cre-mediated recombination removed the floxed neo cassette. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Sst-ires-Flp mouse strain in a publication, please cite the originating article(s) and include JAX stock #028579 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Sst<tm3.1(flpo)Zjh> |
Frozen Mouse Embryo | STOCK Sst<tm3.1(flpo)Zjh>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Sst<tm3.1(flpo)Zjh>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Sst<tm3.1(flpo)Zjh>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Sst<tm3.1(flpo)Zjh>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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