Esrrb-IRES-Cre knock-in mice express a highly-penentrant Cre recombinase in pre-implantation four-cell stage embryos. These mice are useful for studying ubiquitous gene function in embryos between the zygote or two-cell and blastocyst stages of development, being particularly effective in the cases of genes with essential gametic or zygotic functions.
Yaacov Barak, University of Pittsburgh
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Esrrb | estrogen related receptor, beta |
Estrogen related receptor beta, a nuclear receptor, encoded by the Esrrb gene is essential for placental development, an important reprogramming factor in inducing pluripotent stem cells (iPSC), and is involved in germ cell development, inner ear and photoreceptor rod function, and tumorigenesis. This Esrrb-IRES-Cre knock-in strain expresses Cre recombinase from the endogenous Esrrb locus. Cre recombinase activity is detected in pre-implantation embryonic blastomeres, after 2 cleavages, at the onset of the four-cell stage of development.
This Cre deleter strain excises loxP-flanked sequence in all 4 blastomeres in more than 90% of embryos. Heterozygotes are viable and fertile. While mice that are homozygous for the targeted mutation are viable, fertility has not been exhaustively examined. C57BL/6J x 129S1/SvImJ hybrid derived HYB12 embryonic stem (ES) cells were used to generate this strain, with the targeting vector designed using a C57BL/6J template to retain B6 flanking sequence around the mutation.
A targeting vector designed by Dr. Yaacov Barak (University of Pittsburgh, Magee-Womens Research Institute) containing IRES-Cre recombinase-PGK/NEO sequence was inserted into exon 7, at the start of the long 3’ non-coding sequence, simultaneously replacing a portion of the 3’ non-coding sequence. The targeting vector was designed using a C57BL/6J template from a C57BL6J BAC library to retain B6 flanking sequence around the mutation after homologous recombination. The construct was electroporated into C57BL/6J x 129S1/SvImJ hybrid derived HYB12 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then backcrossed to C57BL/6J for 8 generations.
Upon arrival, heterozygous male sperm from was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | pre-implantation embryonic blastomeres |
Allele Name | targeted mutation 1, Yaacov Barak |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Esrrb-IRES-Cre |
Gene Symbol and Name | Esrrb, estrogen related receptor, beta |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | pre-implantation embryonic blastomeres |
Strain of Origin | (C57BL/6J x 129S1/SvImJ)F1 |
Chromosome | 12 |
General Note | ES cell = HYB12 (C57BL/6J x 129S1/SvImJ) |
Molecular Note | A targeting vector containing IRES-Cre recombinase-PGK/NEO sequence was inserted into exon 7, at the start of the long 3' non-coding sequence, simultaneously replacing a portion of the 3' non-coding sequence. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The fertility of homozygotes has not been fully examined.
When using the Esrrb-IRES-Cre knock-in mouse strain in a publication, please cite the originating article(s) and include JAX stock #028575 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Esrrb<tm1(cre)Yba> |
Frozen Mouse Embryo | B6.Cg-Esrrb<tm1(cre)Yba>/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Esrrb<tm1(cre)Yba>/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Esrrb<tm1(cre)Yba>/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Esrrb<tm1(cre)Yba>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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