Estrogen related receptor beta, a nuclear receptor, encoded by the Esrrb gene is essential for placental development, an important reprogramming factor in inducing pluripotent stem cells (iPSC), and is involved in germ cell development, inner ear and photoreceptor rod function, and tumorigenesis. This Esrrb-IRES-Cre knock-in strain expresses Cre recombinase from the endogenous Esrrb locus. Cre recombinase activity is detected in pre-implantation embryonic blastomeres, after 2 cleavages, at the onset of the four-cell stage of development. 
This Cre deleter strain excises loxP-flanked sequence in all 4 blastomeres in more than 90% of embryos. Heterozygotes are viable and fertile. While mice that are homozygous for the targeted mutation are viable, fertility has not been exhaustively examined. C57BL/6J x 129S1/SvImJ hybrid derived HYB12 embryonic stem (ES) cells were used to generate this strain, with the targeting vector designed using a C57BL/6J template to retain B6 flanking sequence around the mutation.

Esrrb-IRES-Cre knock-in mice express a highly-penentrant Cre recombinase in pre-implantation four-cell stage embryos. These mice are useful for studying ubiquitous gene function in embryos between the zygote or two-cell and blastocyst stages of development, being particularly effective in the cases of genes with essential gametic or zygotic functions.

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