These "SncaΔflox" knock-out mice mutant mice lack the first coding exon (exon II) of the Snca (synuclein, alpha) gene. This strain may be useful in studies related to neurodegenerative synucleinopathies, such as Parkinson's disease and multiple system atrophy.
Vladimir L Buchman, Cardiff University School of Biosciences
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Snca | synuclein, alpha |
These "SncaΔflox" knock-out mice mutant mice carry a 1164 bp fragment deletion containing the first coding exon (exon II) and adjacent flanking sequence of the Snca (synuclein, alpha) gene. Mice that are homozygous for the targeted mutation are viable and fertile. No ectopic
sequences with exception of single loxP site at the position of the deleted fragment is present in the
modified locus. No gene product (protein) is detected by Western blot analysis of neural tissue (cerebral cortex and midbrain) from homozygotes.
Mutations in human SNCA that result in aberrant polymerization and fibrillar aggregations are associated with neurodegenerative synucleinopathies, such as Parkinson's disease and multiple system atrophy.
A targeting vector containing a FRT site-flanked NEO cassette and a loxP site was inserted downstream of exon II (the first coding exon) of the targeted Snca gene, and another loxP site was inserted upstream of exon II. This construct was electroporated into C57BL/6N-Atm1Brd-derived JM8A3.N1 embryonic stem (ES) cells. The resulting chimeric animals were bred to C57BL/6J mice for 2 generations and then intercrossed to generate homozygotes. The homozygotes were crossed to transgenic mice (on a congenic C57BL/6 genetic background) expressing FLP recombinase under the control of the human ACTB promoter, generating SncafloxΔneo mice. The FLP recombinase mediated excision of the FRT flanked NEO cassette and a 400 bp fragment encompassing a simple repeat region, which does not affect Snca locus expression. The SncafloxΔneo mice that retained the floxed exon II were bred to C57BL/6J (Charles River) mice for 2 generations to remove the FLP recombinase transgene, before being intercrossed to generate homozygotes. These homozygous SncafloxΔneo/floxΔneo mice were then bred to transgenic mice (on the C57BL/6 background) expressing Cre recombinase under the control of the CMV protomer (see, for example, Stock No. 006054) to excise the floxed exon II and flanking sequences. The resulting knock-out "SncaΔflox" mice were then bred to C57BL/6J (Charles River) for 4 generations to to remove the Cre recombinase transgene, before being intercrossed to generate homozygotes. No ectopic
sequences with exception of single loxP site at the position of the deleted fragment is present in the
modified locus. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1.2, Vladimir L Buchman |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Sncadelta floxed |
Gene Symbol and Name | Snca, synuclein, alpha |
Gene Synonym(s) | |
Strain of Origin | C57BL/6N-Atm1Brd |
Chromosome | 6 |
Molecular Note | A targeting vector containing a FRT site-flanked neo cassette and a loxP site was inserted downstream of exon 2 (the first coding exon) of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed. Germline, Cre-mediated recombination removed exon 2. Western blot analysis of neural tissues confirmed that no protein is expressed. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the SncaΔflox, Snca KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #028559 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Snca<tm1.2Vlb> |
Frozen Mouse Embryo | B6(Cg)-Snca<tm1.2Vlb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Snca<tm1.2Vlb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Snca<tm1.2Vlb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Snca<tm1.2Vlb>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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