Exons 1 and 2 of the mouse Cbfb gene are flanked by loxP sites in this conditional mutant strain. Cre-mediated excision of the floxed region in the Cbfbf mice results in a knockout allele.
David D. Ginty, Harvard Medical School
Cbfb (core binding factor beta) is an essential component of a complex with Runx family transcription factors.
These conditional targeted mutant mice incorporate loxP sites on either side of exons 1 and 2. Cre-mediated excision of the floxed region results in a knockout allele.
Effective gene ablation in the dorsal root ganglia has been demonstrated by crossing these floxed mice with a Wnt1-Cre mouse line, which drives recombination specifically in the dorsal neural tube and neural crest derivatives.
An FRT-flanked neomycin cassette and loxP site were introduced 1 kb upstream of the transcription start site, and a second loxP site was place in intron 2 via homologous recombination in 129S6/SvEvTac-derived embryonic stem (ES) cells. Resultant chimeric animals were crossed with germline FlpE mice (see Stock No. 003800) to excise the neomycin cassette, leaving exons 1 and 2 flanked by loxP sites. This strain was maintained on a mixed and undefined genetic background by the donating laboratory.
|Allele Name||targeted mutation 2.1, David D Ginty|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Cbfb, core binding factor beta|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||An FRT-flanked neomycin cassette and loxP site were introduced 1 kb upstream of the transcription start site, and a second loxP site was place in intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette.|
Heterozygous and homozygous floxed mice are viable and fertile.
When using the Cbfbf mouse strain in a publication, please cite the originating article(s) and include JAX stock #028550 in your Materials and Methods section.