Overview

Also Known As:Cart-IRES2-Cre-D

Cart-IRES2-Cre-D knockin mice have Cre recombinase expression directed to CART prepropeptide-expressing cells, without disrupting endogenous Cartpt expression. These mice may be used to generate conditional mutations for studying gain-or-loss of function and/or fate mapping in subgroups of brain tissues (including cortex, cerebral nuclei, hypothalamus and midbrain) in regards to feeding, appetite and stress response, as well as gene regulation by cocaine and other drugs.

Donating Investigator

Hongkui Zeng, Allen Institute for Brain Science

Genetic overview

Genetic Background	Generation
	N4+pN1F10 (2020-01-20 00:00:00)

Cartpt^{tm1.1(cre)Hze}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Cartpt	CART prepropeptide

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Research Applications

Research Tools
Cancer Research
Neurobiology Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

333.51 Domestic price for breeder pair

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Details

Detailed Description

The Cartpt gene encodes CART prepropeptide isoforms that are processed into multiple biologically active peptides. Expression of this gene is regulated by cocaine and other drugs, and is associated with feeding/appetite and stress response.

The Cart-IRES2-Cre-D knockin mutation has an IRES2 sequence and a Cre recombinase gene inserted downstream of the translational STOP codon of the Cartpt gene. As such, Cart-IRES2-Cre-D mice have both endogenous gene and Cre recombinase expression directed to CART prepropeptide-expressing cells by the endogenous promoter/enhancer elements of the Cartpt locus. When Cart-IRES2-Cre-D mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Cartpt-expressing cells in the offspring.

The donating investigator reports Cre recombinase expression and activity largely recapitulates the endogenous Cartpt gene's unique expression pattern in areas such as cortex, cerebral nuclei, hypothalamus and midbrain.

The donating investigator did not examine cre expression in tissues other than brain.

Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. To date (December 2015), it has not been attempted to make this strain homozygous.

Development

These Cart-IRES2-Cre-D mice (also called Cart-IRES2-Cre-Δ or Cart-IRES2-Cre-Δneo) have an IRES2 sequence and a Cre recombinase gene inserted immediately downstream of the translational STOP codon of the CART prepropeptide gene (Cartpt) on chromosome 13. The specific details are below.

The targeting vector contained, from 5' to 3',
a partial Cartpt sequence spanning exon 1 through intron 2,
an frt3 site within Cartpt intron 2,
a partial Cartpt exon 3 sequence up to and including the endogenous stop codon,
an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence),
a Cre recombinase gene,
a bovine growth hormone polyA sequence,
an AttB site,
a PGK promoter-Neomycin resistance gene-PGK polyA cassette,
an frt5 site,
an mRNA splice acceptor sequence,
the 3' portion of the hygromycin gene (Hygro2) with SV40 late polyA signal,
and an AttP site.

This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice (C57BL/6J congenic background; see Stock No. 007743) to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL).

The resulting Cart-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice for several generations (and the PhiC31 transgene was removed). In 2016, black male mice from generation N4 were sent to The Jackson Laboratory Repository. Upon arrival, males are used to cryopreserve sperm. To establish the living Cartpt-IRES2-Cre-D mouse colony, an aliquot of the frozen sperm is used to fertilize oocytes from C57BL/6J inbred females (Stock No. 000664).

Of note, the donating investigator reports that, at least once during backcrossing, a heterozygous female was bred to a C57BL/6J inbred male (thus the Y chromosome of the congenic strain is of C57BL/6J origin).

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase expression recapitulates the endogenous Cartpt gene's expression in the cortex, cerebral nuclei, hypothalamus and midbrain.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Cartpt^{tm1.1(cre)Hze}

Allele Symbol: Cartpt^{tm1.1(cre)Hze}

Allele Name	targeted mutation 1.1, Hongkui Zeng
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Cart-IRES2-Cre-D
Gene Symbol and Name	Cartpt, CART prepropeptide
Gene Synonym(s)
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase expression recapitulates the endogenous Cartpt gene's expression in the cortex, cerebral nuclei, hypothalamus and midbrain.
Strain of Origin	(129S6/SvEvTac x C57BL/6NCrl)F1
Chromosome	13
Molecular Note	The targeting vector contains, from 5' to 3', a partial Cartpt sequence spanning exon 1 through intron 2, an FRT3 site within Cartpt intron 2, a partial Cartpt exon 3 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an FRT5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 late polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL).

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers: neurons
- Cre-lox System
  - Cre Recombinase Expression
- Neurobiology Research
  - cell marker
Cancer Research
- Cre-lox System
  - Cre recombinase expression
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

References

Additional - Cartpt^{tm1.1(cre)Hze} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Cartpt alternate2
- Genotyping resources and troubleshooting
Dietary Information
- New Diet as of March 2015: Lab Diet® 5K0Q (6% fat)
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). To date (December 2015), it has not been attempted to make this strain homozygous.
- Additional Breeding and Husbandry Support
Mating System
- Wild-type x Heterozygote
- Heterozygote x Wild-type
Citation
When using the Cart-IRES2-Cre-D mouse strain in a publication, please cite the originating article(s) and include JAX stock #028533 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Cartpt<tm1.1(cre)Hze>

Related Products and Services

Frozen Mouse Embryo	B6;129S-Cartpt<tm1.1(cre)Hze>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129S-Cartpt<tm1.1(cre)Hze>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129S-Cartpt<tm1.1(cre)Hze>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6;129S-Cartpt<tm1.1(cre)Hze>/J Frozen Embryo	$3373.50

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Also Known As:Cart-IRES2-Cre-D

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Cartpttm1.1(cre)Hze

Allele Symbol: Cartpttm1.1(cre)Hze

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Cartpttm1.1(cre)Hze related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cartpt^{tm1.1(cre)Hze}

Allele Symbol: Cartpt^{tm1.1(cre)Hze}

Additional - Cartpt^{tm1.1(cre)Hze} related