These floxed mutant mice possess loxP sites flanking exon 3 of the Apoe gene. This strain may be useful for generating conditional mutations in applications related to the regulation of lipoprotein and cholesterol metabolism, neurovascular function, inflammation and in the pathophysiology of neurodegenerative cardiovascular diseases.
Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill
Theodore Mazzone, NorthShore University HealthSystem/University of Chicago
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Apoe | apolipoprotein E |
A major function of apolipoprotein E (APOE) is mediation of lipid transport throughout various tissues and cells. Circulating APOE is associated with chylomicron and Intermediate-density lipoprotein (IDLs) and is mostly produced by the liver, although other tissues and cells, including adrenal gland, macrophages, vascular smooth muscle cells, and adipocytes, are also known to synthesize and secrete APOE.
In the central nervous system, APOE is produced by glial cells, and by injured neurons and cells in the choroid plexus at a lower levels than observed in astrocytes. APOE is important in lipoprotein and cholesterol metabolism, neurovascular function, regulation of the immune system and in the pathophysiology of neurodegenerative diseases such as Alzheimer’s disease and cardiovascular diseases such as atherosclerosis.
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
Removal of the floxed sequence creates a null allele.
When bred to a strain with Cre recombinase expression in adipose tissue (see Stock No. 005069 for example) to generate SEKO (selective apoE knockout- adipose tissue) mice, this mutant mouse strain may be useful in studies of lipid metabolism and adipose tissue inflammation.
A targeting vector designed by Drs. Theodore Mazzone (University of Chicago) and Nobuyo Maeda (University of North Carolina, Chapel Hill) containing a FRT site flanked NEO cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 3. This construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells which were transiently transfected with a FLP recombinase vector to remove the selection cassette. ES cells that had successfully undergone FLP recombination and no longer retained the selection cassette but did retain the loxP-flanked exon 3 were injected in blastocysts. The resulting chimeric animals were tested for germline transmission.
The mice were then backcrossed to C57BL/6J for 10 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1.1, Nobuyo Maeda |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Apoeflox |
Gene Symbol and Name | Apoe, apolipoprotein E |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 7 |
Molecular Note | The targeting vector contains a loxP site and an FRT-flanked neo cassette inserted downstream of exon 3 of the targeted gene, and another loxP site inserted upstream of exon 3. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 3 floxed. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the ApoE-Flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #028530 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Apoe<tm1.1Mae> |
Frozen Mouse Embryo | B6.129S6-Apoe<tm1.1Mae>/MazzJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Apoe<tm1.1Mae>/MazzJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Apoe<tm1.1Mae>/MazzJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Apoe<tm1.1Mae>/MazzJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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