Homozygous CD160 KO mice exhibit an increased susceptibility to inoculation with NK-dependent tumors. These mice may be useful in studying cytokine production by NK cells and NK-dependent tumor growth
Yang-Xin Fu, UT Southwestern
Cd160 encodes a glycosylphosphatidylinositol-anchored protein with a single immunoglobulin-like domain. CD160 has a broad specificity for classical and nonclassical MHC class I molecules as well as herpesvirus entry mediator (HVEM), a TNF family member. Expression is restricted to activated natural killer (NK) and T cells and all intestinal intraepithelial lymphocytes.
The Cd160 knockout allele has a PGK-neo cassette replacing exon 2 of the gene. Homozygous mice are viable and fertile with no reported reproductive deficiency.
Innoculation of homozygous mice with NK-sensitive tumor cells results in increased tumor sizes and reduced NK cell interferon-gamma secretion compared to WT mice. These mice may be useful in studying cytokine production by NK cells and NK-dependent tumor growth.
A targeting vector was designed by Dr. Yang-Xin Fu (University of Chicago) to
replace exon 2 of the Cd160 gene on chromosome 3 with a PGK-neo cassette. Exon 2 contains the initiation codon and is required for all splice variants.
The construct was electroporated into C57BL/6N-derived PRXN embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and the donating investigator reported that the chimeric males were bred with C57BL/6J females to establish the colony (see SNP note below). Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 27 markers throughout the genome were segregating, suggesting an incomplete backcross. Three of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Yang-Xin Fu|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Cd160, CD160 antigen|
|Strain of Origin||C57BL/6NTac|
|Molecular Note||The targeting vector was designed to replace exon 2 with a PGK-neo cassette. Exon 2 contains the initiation codon and is required for all splice variants. Removal of exon 2 forces downstream exons out of frame. Testing by immunohistochemistry confirms the absence of protein.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the CD160- mouse strain in a publication, please cite the originating article(s) and include JAX stock #028527 in your Materials and Methods section.