C57BL/6-Cd160^tm1Yxf/J

Stock number: 028527
Product name: CD160^-
Research areas: Cancer Research, Research Tools

Description

Homozygous CD160 KO mice exhibit an increased susceptibility to inoculation with NK-dependent tumors. These mice may be useful in studying cytokine production by NK cells and NK-dependent tumor growth

Detailed description

Cd160 encodes a glycosylphosphatidylinositol-anchored protein with a single immunoglobulin-like domain. CD160 has a broad specificity for classical and nonclassical MHC class I molecules as well as herpesvirus entry mediator (HVEM), a TNF family member. Expression is restricted to activated natural killer (NK) and T cells and all intestinal intraepithelial lymphocytes. The Cd160 knockout allele has a PGK-neo cassette replacing exon 2 of the gene. Homozygous mice are viable and fertile with no reported reproductive deficiency. Innoculation of homozygous mice with NK-sensitive tumor cells results in increased tumor sizes and reduced NK cell interferon-gamma secretion compared to WT mice. These mice may be useful in studying cytokine production by NK cells and NK-dependent tumor growth.

Development

A targeting vector was designed by Dr. Yang-Xin Fu (University of Chicago) to replace exon 2 of the Cd160 gene on chromosome 3 with a PGK-neo cassette. Exon 2 contains the initiation codon and is required for all splice variants. The construct was electroporated into C57BL/6N-derived PRXN embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and the donating investigator reported that the chimeric males were bred with C57BL/6J females to establish the colony (see SNP note below). Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 27 markers throughout the genome were segregating, suggesting an incomplete backcross. Three of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Cd160^tm1Yxf
Name: targeted mutation 1, Yang-Xin Fu
MGI accession ID: MGI:5698520
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd160
Marker name: CD160 antigen
Chromosome: 3
Strain of origin: C57BL/6NTac
Molecular note: The targeting vector was designed to replace exon 2 with a PGK-neo cassette. Exon 2 contains the initiation codon and is required for all splice variants. Removal of exon 2 forces downstream exons out of frame. Testing by immunohistochemistry confirms the absence of protein.