Mirc32 (associated with colon cancer and other epithelial tumors) is deleted in this miR-143/145Null/+ targeted mutant strain.
Joshua Mendell, UT Southwestern Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Mirc32 | microRNA cluster 32, including Mir143 and Mir145a |
Downregulation of the miR-143/145 cluster (also called Mirc32, microRNA cluster 32, including Mir143 and Mir145a) has been reported in colon cancer and other epithelial tumors. Overexpression of these miRNAs inhibits tumorigenesis, suggesting they function as cell-autonomous epithelial tumor suppressors.
Mirc32 sequences are deleted in this miR-143/145Null/+ null targeted mutant strain. MiR-143/145 microRNA is undetectable in homozygous null mice. Germline deletion of these miRNAs results in no overt developmental defects and the targeted allele is transmitted at expected Mendelian ratios. Animals lacking Mirc32 show normal intestinal development, but epithelial regeneration after injury is dramatically impaired.
Update February, 2019: Although the donating investigator reports no viability or fertility issues, colonies maintained at The Jackson Laboratory demonstrate that homozygotes are generally nonproductive. The few litters produced by homozygotes are very small, with pups often not surviving to weaning age. Heterozygote x heterozygote crosses also produce small litters (~ 5 pups).
A loxP site was inserted upstream of Mir143 and an FRT-flanked neomycin cassette and loxP site were inserted downstream of Mir145a via homologous recombination in a 129-derived embryonic stem (ES) cell line. Crosses with an ACTB or CMV-Cre strain deleted the floxed sequences in the germline. This strain was backcrossed to C57BL/6J for 15 generations by the donating lab.
Allele Name | targeted mutation 1.2, Joshua T Mendell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | miRnull; miR-143/145null |
Gene Symbol and Name | Mirc32, microRNA cluster 32, including Mir143 and Mir145a |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 18 |
Molecular Note | A loxP site was inserted upstream of Mir143 and an FRT flanked neo cassette and loxP site were inserted downstream of Mir145a via homologous recombination. Recombinase mediated recombination removed the neo cassette and both microRNAs. |
Although the donating investigator reports no viability or fertility issues, colonies maintained at The Jackson Laboratory demonstrate that homozygotes are generally nonproductive. The few litters produced by homozygotes are very small, with pups often not surviving to weaning age. Heterozygote x heterozygote crosses also produce small litters (~ 5 pups).
When using the miR-143/145Null/+ mouse strain in a publication, please cite the originating article(s) and include JAX stock #028504 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Mirc32<tm1.2Jtm> |
Frozen Mouse Embryo | B6.129(Cg)-Mirc32<tm1.2Jtm>/J | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Mirc32<tm1.2Jtm>/J | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Mirc32<tm1.2Jtm>/J | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Mirc32<tm1.2Jtm>/J | $3373.50 |
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