This knockout allele causes severely impaired T helper cell (Th2) immune responses, retinal detachment, as well as abnormal insulin signaling and glucose transport. These mice are suitable for use in applications related to T cell development, Th2-mediated disease, asthma, diabetes, serous retinal detachment and late-onset retinal pigment epithelium atrophy.Read More +
Mice homozygous for the Prkcqtm1Litt targeted allele are viable, fertile, normal in size, and do not display any behavioral abnormalities. No endogenous or truncated protein product was detected in thymocytes or T cells. Mature T lymphocytes from null mice have blunted proliferative responses with decreased levels of both IL-2 and IL-2 receptor, and defective T cell receptor-initiated IkappaB-degradation/NF-kappaB activation. Homozygous mice exhibit severely impaired Th2, but normal Th1, immune responses as well as abnormal insulin signaling and glucose transport. Mutant mice also have defective regulatory T cell development (very low CD25 expression). Additionally, homozygotes develop retinal detachments and late-onset retinal pigment epithelium atrophy. This strain lacks the Crb1rd8 mutation that is present in Stock No. 005711 and Stock No. 031293. This mutant may be suitable for use in studies related to T cell proliferation/signal transduction/immunodeficiency, Th2-mediated disease, asthma, diabetes, and exudative retinal detachment. No significant difference in ocular phenotype has been detected between mice homozygous for Prkcqtm1Litt and wild-type at Crb1 with those doubly homozygous for both Prkcqtm1Litt and Crb1rd8.
The B6.129P2-Prkcqtm1Litt/J strain (see Stock No. 005711) was recovered from cryopreservation and found to be homozygous for Crb1rd8. After two cycles of backcross-intercross breeding to C57BL/6J to remove the Crb1rd8 mutation this strain was maintained by sibling intercrossing and fixed homozygous for Crb1+ and Prkcqtm1Litt.
|Allele Name||targeted mutation 1, Dan Littman|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||PKC-Theta-; PKCtheta-|
|Gene Symbol and Name||Prkcq, protein kinase C, theta|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The exon encoding the ATP-binding site of the kinase domain (aa 396 - 451) was replaced with a neo cassette inserted by homologous recombination. Protein was undetected in thymocytes or T cells obtained from mutant mice.|
|Mutations Made By|| |
Dr. Dan Littman, New York University Medical Center