This knockout allele causes severely impaired T helper cell (Th2) immune responses, retinal detachment, as well as abnormal insulin signaling and glucose transport. These mice are suitable for use in applications related to T cell development, Th2-mediated disease, asthma, diabetes, serous retinal detachment and late-onset retinal pigment epithelium atrophy.Read More +
|Allele Type||Gene Symbol||Gene Name|
|Not Applicable||Crb1||crumbs family member 1, photoreceptor morphogenesis associated|
|Allele Type||Gene Symbol||Gene Name|
|Targeted (Null/Knockout)||Prkcq||protein kinase C, theta|
Mice homozygous for the Prkcqtm1Litt targeted allele are viable, fertile, normal in size, and do not display any behavioral abnormalities. No endogenous or truncated protein product was detected in thymocytes or T cells. Mature T lymphocytes from null mice have blunted proliferative responses with decreased levels of both IL-2 and IL-2 receptor, and defective T cell receptor-initiated IkappaB-degradation/NF-kappaB activation. Homozygous mice exhibit severely impaired Th2, but normal Th1, immune responses as well as abnormal insulin signaling and glucose transport. Mutant mice also have defective regulatory T cell development (very low CD25 expression). Additionally, homozygotes develop retinal detachments and late-onset retinal pigment epithelium atrophy. This strain lacks the Crb1rd8 mutation that is present in Stock No. 005711 and Stock No. 031293. This mutant may be suitable for use in studies related to T cell proliferation/signal transduction/immunodeficiency, Th2-mediated disease, asthma, diabetes, and exudative retinal detachment. No significant difference in ocular phenotype has been detected between mice homozygous for Prkcqtm1Litt and wild-type at Crb1 with those doubly homozygous for both Prkcqtm1Litt and Crb1rd8.
The B6.129P2-Prkcqtm1Litt/J strain (see Stock No. 005711) was recovered from cryopreservation and found to be homozygous for Crb1rd8. After two cycles of backcross-intercross breeding to C57BL/6J to remove the Crb1rd8 mutation this strain was maintained by sibling intercrossing and fixed homozygous for Crb1+ and Prkcqtm1Litt.
|Allele Name||wild type|
|Allele Type||Not Applicable|
|Allele Synonym(s)||Crb1+; wild type|
|Gene Symbol and Name||Crb1, crumbs family member 1, photoreceptor morphogenesis associated|
|Gene Synonym(s)||7530426H14Rik; A930008G09Rik; RIKEN cDNA 7530426H14 gene; RIKEN cDNA A930008G09 gene; LCA8; 7530426H14Rik; RP12; A930008G09Rik|
|Strain of Origin||Not Applicable|
|Allele Name||targeted mutation 1, Dan Littman|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Prkcqtm1Litt; targeted mutation 1, Dan Littman|
|Gene Symbol and Name||Prkcq, protein kinase C, theta|
|Gene Synonym(s)||nPKC-theta; Pkcq; A130035A12Rik; A130035A12Rik; PRKCT; PKC theta; Pkcq; PKCtheta; PKC-0; PKC-theta; RIKEN cDNA A130035A12 gene; AW494342; expressed sequence AW494342|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The exon encoding the ATP-binding site of the kinase domain (aa 396 - 451) was replaced with a neo cassette inserted by homologous recombination. Protein was undetected in thymocytes or T cells obtained from mutant mice.|
|Mutations Made By|| |
Dr. Dan Littman, New York University Medical Center
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.