STOCK Tg(tetO-DTA*G128D)2.11Sahay/J

Stock number: 028487
Product name: tet(O)DTA
Research areas: Cell Biology Research, Neurobiology Research, Research Tools

Description

These Ptet-DTA or tet(O)DTA mice express debilitated (tox176 attenuated) diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO) and may be useful in generating bi-transgenic mutant mice for the inducible deletion of specific groups of cells.

Detailed description

These transgenic mice express debilitated (tox176 attenuated) diphtheria toxin A (DTA) under the direction of the tetracycline operator (tetO). When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), diphtheria toxin A expression can be regulated with the tetracycline analog doxycycline in the resulting double mutant offspring. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (March, 2016).

When crossed to transgenic mice expressing the reverse tetracycline-regulated transactivator (tTA) driven by a keratin 5 promoter, inducible ablation of airway basal stem cells is possible in the double transgenic mice that result.



Additionally, when crossed to transgenic mice expressing tTA driven by the mouse Gfap promoter, the resulting double transgenic mice may be used for inducible ablation of GFAP-expressing glial cells.

Development

A transgenic construct containing the debilitated (attenuated) diphtheria toxin A gene, tox176 DTA (DTA*G128D), under the control of the tetO, tetracycline-responsive regulatory element promoter, was injected into fertilized C57BL/6 mouse eggs. Founder line 2.11 was subsequently established. The donating investigator reports that the mice were maintained on the C57BL/6 background (see SNP note below). Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 27 markers throughout the genome were segregating with an unknown strain, suggesting a mixed background. Also, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Tg(tetO-DTA*G128D)2.11Sahay
Name: transgene insertion 2.11, Amar Sahay
MGI accession ID: MGI:5779909
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-DTA*G128D)2.11Sahay
Marker name: transgene insertion 2.11, Amar Sahay
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Dta,Diphtheria toxin A chain,
Molecular note: The transgenic construct contains the debilitated (attenuated) diphtheria toxin A gene, tox176 DTA (DTA*G128D), under the control of the tetO, tetracycline-responsive regulatory element promoter.