These Ptet-DTA or tet(O)DTA mice express debilitated (tox176 attenuated) diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO) and may be useful in generating bi-transgenic mutant mice for the inducible deletion of specific groups of cells.
Amar Sahay, Massachusetts General Hospital, Harvard Medical School
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
These transgenic mice express debilitated (tox176 attenuated) diphtheria toxin A (DTA) under the direction of the tetracycline operator (tetO).
When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), diphtheria toxin A expression can be regulated with the tetracycline analog doxycycline in the resulting double mutant offspring. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (March, 2016).
When crossed to transgenic mice expressing the reverse tetracycline-regulated transactivator (tTA) driven by a keratin 5 promoter, inducible ablation of airway basal stem cells is possible in the double transgenic mice that result.
Additionally, when crossed to transgenic mice expressing
tTA driven by the mouse Gfap promoter, the resulting double transgenic mice may be used for inducible ablation of GFAP-expressing glial cells.
A transgenic construct containing the debilitated (attenuated) diphtheria toxin A gene, tox176 DTA (DTA*G128D), under the control of the tetO, tetracycline-responsive regulatory element promoter, was injected into fertilized C57BL/6 mouse eggs. Founder line 2.11 was subsequently established. The donating investigator reports that the mice were maintained on the C57BL/6 background (see SNP note below).
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 27 markers throughout the genome were segregating with an unknown strain, suggesting a mixed background. Also, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | Dta, Diphtheria toxin A chain, |
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Site of Expression |
Allele Name | transgene insertion 2.11, Amar Sahay |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | Ptet-DTA |
Gene Symbol and Name | Tg(tetO-DTA*G128D)2.11Sahay, transgene insertion 2.11, Amar Sahay |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Dta, Diphtheria toxin A chain, |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The transgenic construct contains the debilitated (attenuated) diphtheria toxin A gene, tox176 DTA (DTA*G128D), under the control of the tetO, tetracycline-responsive regulatory element promoter. |
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous. Homozygous viability/fertility has not been tested (March, 2016).
When using the tet(O)DTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #028487 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(tetO-DTA*G128D)2.11Sahay |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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