Vclfl/fl floxed mice possess loxP sites flanking exon 3 of the vinculin gene making this strain useful for studying the regulation of cell-cell and cell-matrix adhesion.
Robert Ross, University of California, San Diego
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Vcl | vinculin |
Vclfl/fl floxed mice possess loxP sites flanking exon 3 of the vinculin (Vcl) gene. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is involved in anchoring actin filaments to the cell membrane. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
When bred to mice expressing Cre Recombinase in cardiac myocytes, Vcl deletion results in a disruption of the adherens junctions in the myocardium, causing death by 3 months of age by either sudden death or dilated cardiomyopathy with progressive heart failure.
A targeting vector was designed by Dr Robert Ross (University of California, San Diego School of Medicine) to insert a loxP site upstream of exon 3, and a second loxP site downstream of exon 3 of the vinculin (Vcl) gene. A frt-flanked neomycin resistance (neo) cassette was placed downstream of the 3’ loxP site. The construct was electroporated into 129S4/SvJae-derived LW1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with
B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703) transgenic mice to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene, and resulting Vclfl/fl mice were bred to Black Swiss mice. Subsequently, mice were bred to C57BL/6J mice for 15 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1, Robert S Ross |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Vclfl; Vclflx |
Gene Symbol and Name | Vcl, vinculin |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 14 |
Molecular Note | An frt-flanked neo cassette with a 5' loxP site was inserted downstream of exon 3. An additional loxP site was inserted upstream of exon 3. Flp-mediated recombination was used to remove the neo cassette leaving a floxed exon 3. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Vclfl mouse strain in a publication, please cite the originating article(s) and include JAX stock #028451 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Vcl<tm1Ross> |
Frozen Mouse Embryo | B6.129S4(Cg)-Vcl<tm1Ross>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4(Cg)-Vcl<tm1Ross>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4(Cg)-Vcl<tm1Ross>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4(Cg)-Vcl<tm1Ross>/J Frozen Embryo | $3373.50 |
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