Slick floxed mice possess loxP sites flanking exon 22 of the Kcnt2 gene, making this strain useful for studying the regulation of neuronal excitability mutations in Slick channels.
Christopher J. Lingle, Washington University School of Medicine
Slick floxed mice possess loxP sites flanking exon 22 of the potassium channel, subfamily T, member 2 (Kcnt2) gene. KCNT2 encodes a subunit of a sodium activated potassium channel expressed in neurons throughout the brain. Slick floxed mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 22 of the Kcnt2 gene deleted in cre-expressing tissues.
A targeting vector was designed by Dr. Xiao-Ming Xia (laboratory of Dr. Christopher Lingle, Washington University School of Medicine) to insert a loxP site upstream of exon 22 of the potassium channel, subfamily T, member 2 (Kcnt2) gene. The construct also inserted a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette which included a third loxP site, downstream of exon 22. The construct was electroporated into C57BL/6-derived BLU embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ mice (Stock No. 009086) to delete the neo cassette. Resulting progeny were crossed to remove the Flp-expressing transgene, and Slick floxed offspring were backcrossed to C57BL/6 mice for at least 15 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, Christopher J Lingle|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Slick floxed|
|Gene Symbol and Name||Kcnt2, potassium channel, subfamily T, member 2|
|Strain of Origin||C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley|
|Molecular Note||A targeting vector was designed to insert a loxP site upstream of exon 22 and a second loxP site downstream of exon22, followed by an FRT-flanked neomycin resistance (neo) cassette which includes a third loxP site. FLP-mediated recombination removed the FRT-flanked neo cassette leaving exon 22 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Slick floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #028419 in your Materials and Methods section.