B6.Cg-Tg(Adipoq-LPL)1Fnln/J

Stock number: 028400
Product name: AdipoQ-LpL
Research areas: Cardiovascular Research, Endocrine Deficiency Research, Internal/Organ Research, Metabolism Research

Description

AdipoQ-LpL mice express FLAG-tagged human lipoprotein lipase specifically in white adipose tissue. These mice are useful when studying lipid accumulation and distribution.

Detailed description

AdipoQ-LpL transgenic mice express FLAG-tagged human lipoprotein lipase (LPL) under the control of the murine adiponectin (Adipoq) promoter. LPL is a lipase synthesized by heart, muscle, and adipose tissue. It is transported to the capillary endothelium where it catalyzes the hydrolysis of triglycerides. LPL delivers fatty acids to adipose tissue for storage or the heart and skeletal muscle as fuel. When adipose lipid storage is exceeded, this lipid is stored in liver and muscle where it contributes to insulin resistance, inflammation, and endoplasmic reticulum stress. Mice hemizygous for the transgene are viable and fertile. The AdipoQ-LpL transgene is expressed specifically in white adipose tissue. These mice have improved glucose and insulin tolerance as well as increased energy expenditure and food intake when challenged with a high fat diet (HFD). They have increased expression of PPARγ and PPARγ-regulated genes in epididymal fat pads. AdipoQ-LpL mice also have increased triglycerides in liver, did not have reduced diacylglycerides or ceramides in liver or muscle. The adipose phenotype of these animals resembles thiazolidinedione (TZD) treatment except there is no increase in weight or adipose mass.

Development

The AdipoQ-LpL transgene was designed by Brian Finlin (University of Kentucky) with the murine adiponectin (Adipoq) 5.4-kb promoter driving expression of a FLAG-tagged human lipoprotein lipase (LPL) gene. The transgene was microinjected into C3H/C57BL/6 fertilized oocytes, and founder mice were bred to C57BL/6J mice to establish a colony. AdipoQ-LpL mice were further backcrossed to C57BL/6J mice for at least 10 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(Adipoq-LPL)1Fnln
Name: transgene insertion 1, Brian Finlin
MGI accession ID: MGI:5699372
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Adipoq-LPL)1Fnln
Marker name: transgene insertion 1, Brian Finlin
Chromosome: UN
Strain of origin: C3H x C57BL/6
Expressed genes: LPL,lipoprotein lipase,human
Molecular note: The AdipoQ-LpL transgene was designed with the murine adiponectin (Adipoq) 5.4-kb promoter driving expression of a FLAG-tagged human lipoprotein lipase (LPL) gene. The transgene is expressed specifically in white adipose tissue.