AdipoQ-LpL mice express FLAG-tagged human lipoprotein lipase specifically in white adipose tissue. These mice are useful when studying lipid accumulation and distribution.
Brian Finlin, University of Kentucky
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
AdipoQ-LpL transgenic mice express FLAG-tagged human lipoprotein lipase (LPL) under the control of the murine adiponectin (Adipoq) promoter.
LPL is a lipase synthesized by heart, muscle, and adipose tissue. It is transported to the capillary endothelium where it catalyzes the hydrolysis of triglycerides. LPL delivers fatty acids to adipose tissue for storage or the heart and skeletal muscle as fuel. When adipose lipid storage is exceeded, this lipid is stored in liver and muscle where it contributes to insulin resistance, inflammation, and endoplasmic reticulum stress. Mice hemizygous for the transgene are viable and fertile. The AdipoQ-LpL transgene is expressed specifically in white adipose tissue. These mice have improved glucose and insulin tolerance as well as increased energy expenditure and food intake when challenged with a high fat diet (HFD). They have increased expression of PPARγ and PPARγ-regulated genes in epididymal fat pads. AdipoQ-LpL mice also have increased triglycerides in liver, did not have reduced diacylglycerides or ceramides in liver or muscle. The adipose phenotype of these animals resembles thiazolidinedione (TZD) treatment except there is no increase in weight or adipose mass.
The AdipoQ-LpL transgene was designed by Brian Finlin (University of Kentucky) with the murine adiponectin (Adipoq) 5.4-kb promoter driving expression of a FLAG-tagged human lipoprotein lipase (LPL) gene. The transgene was microinjected into C3H/C57BL/6 fertilized oocytes, and founder mice were bred to C57BL/6J mice to establish a colony. AdipoQ-LpL mice were further backcrossed to C57BL/6J mice for at least 10 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | LPL, lipoprotein lipase, human |
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Site of Expression |
Allele Name | transgene insertion 1, Brian Finlin |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Adipoq-LPL |
Gene Symbol and Name | Tg(Adipoq-LPL)1Fnln, transgene insertion 1, Brian Finlin |
Gene Synonym(s) | |
Promoter | Adipoq, adiponectin, C1Q and collagen domain containing, mouse, laboratory |
Expressed Gene | LPL, lipoprotein lipase, human |
Strain of Origin | C3H x C57BL/6 |
Chromosome | UN |
Molecular Note | The AdipoQ-LpL transgene was designed with the murine adiponectin (Adipoq) 5.4-kb promoter driving expression of a FLAG-tagged human lipoprotein lipase (LPL) gene. The transgene is expressed specifically in white adipose tissue. |
When maintaining a live colony, hemiygous mice may be bred together. The donating investigator did not attempt to make a homozygous colony.
When using the AdipoQ-LpL mouse strain in a publication, please cite the originating article(s) and include JAX stock #028400 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Adipoq-LPL)1Fnln |
Frozen Mouse Embryo | B6.Cg-Tg(Adipoq-LPL)1Fnln/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Adipoq-LPL)1Fnln/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Adipoq-LPL)1Fnln/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Adipoq-LPL)1Fnln/J Frozen Embryo | $3373.50 |
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