Homozygous female Sycp2l knockout mice exhibit a progressive loss of oocytes and decreased fertility. These mice may be useful in the study of female reproductive aging.
Jeremy Wang, University of Pennsylvania
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Sycp2l | synaptonemal complex protein 2-like |
SYCP2L (synaptonemal complex protein 2-like) is a sequence homolog of SYCP2 and is expressed only in oocytes. Variants in human SYCP2L are associated with Age at Natural Menopause (ANM). SYCP2L localizes to the centromere of dictyate oocytes. The Sycp2l knockout allele has a loxP-flanked PGK neo cassette replacing exons 8-14. Homozygous female mice undergo a progressive reduction of ooctyes with aging; by 14 months oocyte numbers drop to 20% of controls. Primordial follicle number decreases beginning at 2 months of age; decreases in numbers of primary and secondary follicles are apparent at 12 months of age. Although homozygous female mice produce the same number of litters as controls, litter size is significantly decreased. Male fertility is unaffected. These mice may be useful in the study of female reproductive aging.
A targeting vector was designed by Dr. Jeremy Wang (University of Pennsylvania) to
replace exons 8-14 of the synaptonemal complex protein 2-like gene (Sycp2l) on chromosome 13 with a loxP-flanked PGK-neo cassette leading to a frameshift in the transcript.
The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony.
The resulting mice were bred with C57BL/6 wildtype mice for one generation prior to sending males to The Jackson Laboratory Repository in 2015.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1, Jeremy Wang |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Sycp2l, synaptonemal complex protein 2-like |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 13 |
Molecular Note | The targeting vector was designed to replace exons 8-14 of the synaptonemal complex protein 2-like gene (Sycp2l) on chromosome 13 with a loxP-flanked PGK-neo cassette. The absence of protein expression was confirmed by Western blot analysis and immunostaining on ovarian extracts. |
When maintaining a live colony, heterozygous mice may be bred together, or homozygous males to heterozygous females. Homozygous female mice exhibit reduced fertility.
When using the Sycp2l- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41440 in your Materials and Methods section.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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