Overview

Also Known As:Sycp2l^-

Homozygous female Sycp2l knockout mice exhibit a progressive loss of oocytes and decreased fertility. These mice may be useful in the study of female reproductive aging.

Donating Investigator

Jeremy Wang, University of Pennsylvania

Genetic overview

Genetic Background	Generation

Sycp2l^tm1Jw

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Sycp2l	synaptonemal complex protein 2-like

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Research Applications

Reproductive Biology Research

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Details

Detailed Description

SYCP2L (synaptonemal complex protein 2-like) is a sequence homolog of SYCP2 and is expressed only in oocytes. Variants in human SYCP2L are associated with Age at Natural Menopause (ANM). SYCP2L localizes to the centromere of dictyate oocytes. The Sycp2l knockout allele has a loxP-flanked PGK neo cassette replacing exons 8-14. Homozygous female mice undergo a progressive reduction of ooctyes with aging; by 14 months oocyte numbers drop to 20% of controls. Primordial follicle number decreases beginning at 2 months of age; decreases in numbers of primary and secondary follicles are apparent at 12 months of age. Although homozygous female mice produce the same number of litters as controls, litter size is significantly decreased. Male fertility is unaffected. These mice may be useful in the study of female reproductive aging.

Development

A targeting vector was designed by Dr. Jeremy Wang (University of Pennsylvania) to
replace exons 8-14 of the synaptonemal complex protein 2-like gene (Sycp2l) on chromosome 13 with a loxP-flanked PGK-neo cassette leading to a frameshift in the transcript.

The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony.

The resulting mice were bred with C57BL/6 wildtype mice for one generation prior to sending males to The Jackson Laboratory Repository in 2015.

Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Zhou J; Stein P; Leu NA; Chmatal L; Xue J; Ma J; Huang X; Lampson MA; Schultz RM; Wang PJ. 2015. Accelerated reproductive aging in females lacking a novel centromere protein SYCP2L. Hum Mol Genet 24(22):6505-14PubMed: 26362258 MGI: J:225272

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Genetics

Sycp2l^tm1Jw

Allele Symbol: Sycp2l^tm1Jw

Allele Name	targeted mutation 1, Jeremy Wang
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Sycp2l, synaptonemal complex protein 2-like
Gene Synonym(s)
Strain of Origin	(C57BL/6 x 129S4/SvJae)F1
Chromosome	13
Molecular Note	The targeting vector was designed to replace exons 8-14 of the synaptonemal complex protein 2-like gene (Sycp2l) on chromosome 13 with a loxP-flanked PGK-neo cassette. The absence of protein expression was confirmed by Western blot analysis and immunostaining on ovarian extracts.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Reproductive Biology Research
- Fertility Defects
  - females only

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Sycp2l^tm1Jw/Sycp2l^tm1Jw
involves: 129S4/SvJae * C57BL/6

reproductive system phenotype

early reproductive senescence
- females show accelerated reproductive aging

reduced female fertility

decreased litter size
- litter sizes are reduced , however number of litters is similar to controls

decreased oocyte number
- oocyte numbers are similar to controls at 10 days of age, but drop to 20% of controls at 14 months
- progressive reduction of primordial oocytes as compared to controls

decreased primordial ovarian follicle number
- number of primordial follicles is reduced at 2 months, 8 months and 12 months as compared to controls

decreased secondary ovarian follicle number
- number of secondary follicles is reduced at 12 months of age as compared to controls

decreased primary ovarian follicle number
- number of primary follicles is reduced at 12 months of age as compared to controls

cellular phenotype

decreased oocyte number
- progressive reduction of primordial oocytes as compared to controls
- oocyte numbers are similar to controls at 10 days of age, but drop to 20% of controls at 14 months

endocrine/exocrine gland phenotype

decreased primary ovarian follicle number
- number of primary follicles is reduced at 12 months of age as compared to controls

decreased primordial ovarian follicle number
- number of primordial follicles is reduced at 2 months, 8 months and 12 months as compared to controls

decreased secondary ovarian follicle number
- number of secondary follicles is reduced at 12 months of age as compared to controls

mortality/aging

early reproductive senescence
- females show accelerated reproductive aging

References

Zhou J; Stein P; Leu NA; Chmatal L; Xue J; Ma J; Huang X; Lampson MA; Schultz RM; Wang PJ. 2015. Accelerated reproductive aging in females lacking a novel centromere protein SYCP2L. Hum Mol Genet 24(22):6505-14PubMed: 26362258 MGI: J:225272

Additional - Sycp2l^tm1Jw related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Sycp2l
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, or homozygous males to heterozygous females. Homozygous female mice exhibit reduced fertility.
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x Heterozygote
Citation
When using the Sycp2l^- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41440 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Sycp2l-

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Sycp2ltm1Jw

Allele Symbol: Sycp2ltm1Jw

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Sycp2ltm1Jw/Sycp2ltm1Jwinvolves: 129S4/SvJae * C57BL/6

reproductive system phenotype

cellular phenotype

endocrine/exocrine gland phenotype

mortality/aging

References

Additional - Sycp2ltm1Jw related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:Sycp2l^-

Sycp2l^tm1Jw

Allele Symbol: Sycp2l^tm1Jw

Genotype: Sycp2l^tm1Jw/Sycp2l^tm1Jw
involves: 129S4/SvJae * C57BL/6

Additional - Sycp2l^tm1Jw related