These floxed mutant mice possess loxP sites flanking exon 6 of the Tgfb3 gene. This strain may be useful for generating conditional mutations in applications related to TGF-β3 signaling.
Thomas Doetschman, University of Arizona
One of 3 isoforms of the transforming growth factor-β gene family, the protein encoded by transforming growth factor, beta 3 (Tgfb3) is important in embryogenesis, epithelial-mesenchymal transition and cell differentiation, migration and proliferation. These Tgfb3flox
mice possess loxP sites on either side of exon 6 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 6 deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele.
When bred to a strain with germ-line Cre recombinase expression in the early embryo (see Stock No. 003724), the resulting mice die at birth from cleft palate.
A loxP site and a targeting vector containing an FRT flanked MC1NeopA selection cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 6 of the targeted gene, and another loxP site was inserted downstream of exon 6. This construct was electroporated into 129S6/SvEvTac derived KG-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 x DBA2 blastocysts.
Resulting chimeric male animals were backcrossed to wildtype Black-Swiss female mice.
The mice were then crossed to C57BL/6J mice for two generations.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Mohamad Azhar|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Tgfb3, transforming growth factor, beta 3|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A loxP site and an FRT flanked MC1-neomycin selection cassette were inserted in intron 5 and a second loxP site was placed in intron 6.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Tgfb3flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #028359 in your Materials and Methods section.