STOCK Fzd10^tm1.1Nat/J

Stock number: 028317
Product name: Fzd10-CKO-AP
Research areas: Research Tools

Description

Fzd10 coding region sequences are flanked by loxP sites in this Fzd10-CKO-AP allele. Cre-mediated recombination of the floxed region creates a knockout of the gene and enables expression of the alkaline phosphatase coding region under the control of the Fzd10 promoter.

Detailed description

Fzd10 (frizzled homolog 10 (Drosophila)) coding region sequences are flanked by loxP sites in this Fzd10-CKO-AP allele. Cre-mediated recombination of the floxed region creates a knockout of the gene and enables expression of the alkaline phosphatase coding region under the control of the Fzd10 promoter.

Homozygous floxed mice have no apparent phenotype and are healthy and fertile. Expression of Fzd10 mRNA and protein have not been examined directly. Expression of the conditional reporter (alkaline phosphatase) has been examined histochemically.

Development

LoxP sites were inserted (1) within the first coding exon 5’ of the initiator methionine codon, and (2) 3’ of an SV40 polyadenylation signal that was inserted immediately 3’ of the 3’ UTR. The following DNA segments were added immediately downstream of the 3’ loxP site: (1) the coding region for human placental alkaline phosphatase (AP), and (2) a phosphoglycerate kinase (PGK)-Neo cassette flanked by FRT sites (FNF). The targeting construct was electroporated into 129-derived ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were crossed to C57BL/6 mice and then germline Flp mice (see Stock No. 003800) to excise the FRT-PGK-Neo-FRT (FNF) cassette. This strain was maintained on a mixed C57BL/6 - 129 genetic background by the donating lab.

Allele

Symbol: Fzd10^tm1.1Nat
Name: targeted mutation 1.1, Jeremy Nathans
MGI accession ID: MGI:5688883
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter; No functional change
Marker symbol: Fzd10
Marker name: frizzled class receptor 10
Chromosome: 5
Strain of origin: 129
Expressed genes: ALPP,alkaline phosphatase, placental,human
Molecular note: LoxP sites were inserted within the first coding exon 5' of the initiator methionine codon, and 3' of an SV40 polyadenylation signal that was inserted immediately 3' of the 3' UTR. The following DNA segments were added immediately downstream of the 3' loxP site: (1) the coding region for human placental alkaline phosphatase (AP), and (2) a phosphoglycerate kinase (PGK)-Neo cassette flanked by FRT sites (FNF). Flp-mediated recombination removed the FRT-flanked neo cassette.