Fzd10 coding region sequences are flanked by loxP sites in this Fzd10-CKO-AP allele. Cre-mediated recombination of the floxed region creates a knockout of the gene and enables expression of the alkaline phosphatase coding region under the control of the Fzd10 promoter.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, No functional change) | Fzd10 | frizzled class receptor 10 |
Fzd10 (frizzled homolog 10 (Drosophila)) coding region sequences are flanked by loxP sites in this Fzd10-CKO-AP allele. Cre-mediated recombination of the floxed region creates a knockout of the gene and enables expression of the alkaline phosphatase coding region under the control of the Fzd10 promoter.
Homozygous floxed mice have no apparent phenotype and are healthy and fertile. Expression of Fzd10 mRNA and protein have not been examined directly. Expression of the conditional reporter (alkaline phosphatase) has been examined histochemically.
LoxP sites were inserted (1) within the first coding exon 5’ of the initiator methionine codon, and (2) 3’ of an SV40 polyadenylation signal that was inserted immediately 3’ of the 3’ UTR. The following DNA segments were added immediately downstream of the 3’ loxP site: (1) the coding region for human placental alkaline phosphatase (AP), and (2) a phosphoglycerate kinase (PGK)-Neo cassette flanked by FRT sites (FNF). The targeting construct was electroporated into 129-derived ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were crossed to C57BL/6 mice and then germline Flp mice (see Stock No. 003800) to excise the FRT-PGK-Neo-FRT (FNF) cassette. This strain was maintained on a mixed C57BL/6 - 129 genetic background by the donating lab.
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
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Site of Expression |
Allele Name | targeted mutation 1.1, Jeremy Nathans |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, No functional change) |
Allele Synonym(s) | Fzd10-CKO-AP |
Gene Symbol and Name | Fzd10, frizzled class receptor 10 |
Gene Synonym(s) | |
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
Strain of Origin | 129 |
Chromosome | 5 |
Molecular Note | LoxP sites were inserted within the first coding exon 5' of the initiator methionine codon, and 3' of an SV40 polyadenylation signal that was inserted immediately 3' of the 3' UTR. The following DNA segments were added immediately downstream of the 3' loxP site: (1) the coding region for human placental alkaline phosphatase (AP), and (2) a phosphoglycerate kinase (PGK)-Neo cassette flanked by FRT sites (FNF). Flp-mediated recombination removed the FRT-flanked neo cassette. |
Homozygous and heterozygous mice are viable and fertile.
When using the Fzd10-CKO-AP mouse strain in a publication, please cite the originating article(s) and include JAX stock #028317 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Fzd10<tm1.1Nat> |
Frozen Mouse Embryo | STOCK Fzd10<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Fzd10<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Fzd10<tm1.1Nat>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Fzd10<tm1.1Nat>/J | $3373.50 |
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