B6.129-Syn2^tm1Pggd/Mmjax

Stock number: 028252
Product name: synapsin II KO
Research areas: Neurobiology Research

Description

Homozygous synapsin II knockout mice exhibit seizures and retarded axon formation. These mice may be useful in studying the role of synapsin II in neurodevelopment.

Detailed description

TheSyn2 gene is a member of the synapsin family of neuronal phophsoproteins, which are associated with neurotransmitter release and synaptogenesis. Synapsin proteins are associated with autism. Synapsin II localizes to presynaptic terminals. The synapsin II knockout allele has a PGK-neo cassette replacing exon 1 of the Syn2 gene. Homozygous mice exhibit decreased brain weight, impaired lamellipodia formation, abnormal neurite formation, aberrant actin filament distribution, delayed axon differentiation, altered cytoskeletal formation, and to a lesser extent than the synapsin I KO, delayed synapse formation. Beginning at two months of age, mice experience spontaneous seizures. Homozygous mice are viable and fertile with no reported reproductive deficiency.

Development

A targeting vector was designed by Dr. Paul Greengard (Rockefeller University) to replace exon 1 of the synapsin II gene (Syn2) on chromosome 6 with a PGK-neomycin cassette. The construct was electroporated into 129-derived unspecified embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony. The resulting mice were bred with C57BL/6 wildtype mice for at least ten generations prior to sending males to The Jackson Laboratory Repository in 2015.

Allele

Symbol: Syn2^tm1Pggd
Name: targeted mutation 1, Paul Greengard
MGI accession ID: MGI:3045984
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Syn2
Marker name: synapsin II
Chromosome: 6
Strain of origin: 129
Molecular note: Exon1 was replaced with a neomycin cassette inserted by homologous recombination.