Homozygous synapsin I knockout mice exhibit seizures, delayed axonal outgrowth and delayed synapse formation. These mice may be useful in studying the role of synapsin I in neurodevelopment.
Hung-Teh Kao, Nathan Kline Institute
Paul Greengard, Rockefeller University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Syn1 | synapsin I |
Syn1, the most abundant member of the synapsin family of neuronal phophoproteins, is associated with neurotransmitter release and synaptogenesis. Synapsin I localizes to presynaptic terminals. Synapsin proteins are associated with autism. The synapsin I knockout allele has a PGK-neo cassette replacing exon 1 of the Syn1 gene. Homozygous mice exhibit a delay in the outgrowth of presynaptic neurites, axonal elongation and synapse formation in hippocampal neurons. Beginning at two months of age, mice experience spontaneous seizures. Homozygous mice are viable and fertile with no reported reproductive deficiency.
A targeting vector was designed by Dr. Paul Greengard (Rockefeller University) to
replace exon 1 of the synapsin I gene (Syn1) on chromosome X with a PGK-neomycin cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony. The resulting mice were bred with C57BL/6 wildtype mice for at least ten generations prior to sending males to The Jackson Laboratory Repository in 2015.
Allele Name | targeted mutation 1, Paul Greengard |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Syn1tm1Lli; SynI- |
Gene Symbol and Name | Syn1, synapsin I |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | X |
Molecular Note | Exon 1 was replaced by a neomycin selection cassette inserted by homologous recombination. Western blot analysis of homozygous mutant brain tissues indicated an absence of both protein isoforms, Synapsin Ia and Synapsin Ib, produced by the endogenous locus. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Syn 1 KO mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41436 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.