Homozygous synapsin III knockout mice exhibit altered synaptic vesicle trafficking, reduced synaptic depression and delayed axon outgrowth. These mice may be useful in studying the role of synapsin III in neurodevelopment.
Hung-Teh Kao, Nathan Kline Institute
Paul Greengard, Rockefeller University
The Syn3 gene is a member of the synapsin family of neuronal phosphoproteins, which are associated with neurotransmitter release and neurite outgrowth. Synapsin proteins are associated with autism. Synapsin III localizes to cell bodies, growth cones and presynaptic sites, in contrast to synapsin I and II, which localize only to presynaptic terminals.
The synapsin III knockout allele has a neo cassette replacing exon 1 of the Syn3 gene.
Homozygous mice exhibit an increase in the recycling pool of synaptic vesicles, a reduction in the amplitude of inhibitory postsynaptic currents (IPSC) and a delay in early axon extension.
Homozygous mice are viable and fertile with no reported reproductive deficiency.
A targeting vector was designed by Dr. Paul Greengard (Rockefeller University) to
replace exon 1 of the synapsin 3 gene (Syn3) on chromosome 10 with a neomycin cassette in reverse orientation. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony. The resulting mice were bred with C57BL/6 wildtype mice for at least ten generations.
|Allele Name||targeted mutation 1, Paul Greengard|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Syn3tm1Jfe; synapsin III -|
|Gene Symbol and Name||Syn3, synapsin III|
|Strain of Origin||129X1/SvJ|
|Molecular Note||A neomycin selection cassette replaced exon 1. Western blot analysis on brain extracts derived from homozygous mice confirmed that no detectable protein was made from this allele.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the synapsin III KO mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41435 in your Materials and Methods section.
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