Overview

Also Known As:synapsin III KO

Homozygous synapsin III knockout mice exhibit altered synaptic vesicle trafficking, reduced synaptic depression and delayed axon outgrowth. These mice may be useful in studying the role of synapsin III in neurodevelopment.

Donating Investigator

Hung-Teh Kao, Nathan Kline Institute

Paul Greengard, Rockefeller University

Genetic overview

Genetic Background	Generation

Syn3^tm1Pggd

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Syn3	synapsin III

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Research Applications

Neurobiology Research

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Details

Detailed Description

The Syn3 gene is a member of the synapsin family of neuronal phosphoproteins, which are associated with neurotransmitter release and neurite outgrowth. Synapsin proteins are associated with autism. Synapsin III localizes to cell bodies, growth cones and presynaptic sites, in contrast to synapsin I and II, which localize only to presynaptic terminals.

The synapsin III knockout allele has a neo cassette replacing exon 1 of the Syn3 gene.
Homozygous mice exhibit an increase in the recycling pool of synaptic vesicles, a reduction in the amplitude of inhibitory postsynaptic currents (IPSC) and a delay in early axon extension.
Homozygous mice are viable and fertile with no reported reproductive deficiency.

Development

A targeting vector was designed by Dr. Paul Greengard (Rockefeller University) to
replace exon 1 of the synapsin 3 gene (Syn3) on chromosome 10 with a neomycin cassette in reverse orientation. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony. The resulting mice were bred with C57BL/6 wildtype mice for at least ten generations.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Feng J; Chi P; Blanpied TA; Xu Y; Magarinos AM; Ferreira A; Takahashi RH; Kao HT; McEwen BS; Ryan TA; Augustine GJ; Greengard P. 2002. Regulation of neurotransmitter release by synapsin III. J Neurosci 22(11):4372-80PubMed: 12040043 MGI: J:77048

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Genetics

Syn3^tm1Pggd

Allele Symbol: Syn3^tm1Pggd

Allele Name	targeted mutation 1, Paul Greengard
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Syn3^tm1Jfe; synapsin III -
Gene Symbol and Name	Syn3, synapsin III
Gene Synonym(s)
Strain of Origin	129X1/SvJ
Chromosome	10
Molecular Note	A neomycin selection cassette replaced exon 1. Western blot analysis on brain extracts derived from homozygous mice confirmed that no detectable protein was made from this allele.

Disease/Phenotype

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

autism spectrum disorder

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Neurodevelopmental Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Syn3^tm1Pggd/Syn3^tm1Pggd
involves: 129X1/SvJ

cellular phenotype

delayed axon extension
- observed in hippocampal neurons in culture

nervous system phenotype

abnormal inhibitory postsynaptic currents
- a reduction in amplitude was noted

delayed axon extension
- observed in hippocampal neurons in culture

abnormal excitatory postsynaptic currents
- a slightly larger amplitude was noted

increased synaptic depression
- time constants for depression range is increased two fold
- reduced transmission noted after a 5 second stimulation in homozygotes

The following phenotype relates to a compound genotype created using this strain

Genotype: Syn3^tm1Pggd/Syn3^tm1Pggd
B6.129X1-Syn3

behavior/neurological phenotype

abnormal social investigation
- Normal - however, mutants exhibit normal anxiety related behaviors
- in the resident-intruder test, mutants show reduced social interaction with the intruder mice after 1-2 months of social isolation
- in the social novelty test, young mutants do not discriminate between familiar and novel mice compared to controls that spend more time exploring the novel mouse than a familiar mouse indicating altered social novelty preference

impaired social transmission of food preference
- in the social transmission of food preference task, mutants show an absence of preference for the cued food over the novel food after one hour after social interaction with the demonstrator cagemate

decreased aggression towards mice
- in the social dominance tube test, adults show more submissive behavior than controls

mammalian phenotype

taste/olfaction phenotype
- Normal - olfaction is intact

References

Feng J; Chi P; Blanpied TA; Xu Y; Magarinos AM; Ferreira A; Takahashi RH; Kao HT; McEwen BS; Ryan TA; Augustine GJ; Greengard P. 2002. Regulation of neurotransmitter release by synapsin III. J Neurosci 22(11):4372-80PubMed: 12040043 MGI: J:77048

Additional - Syn3^tm1Pggd related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Syn3-alternate2
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the synapsin III KO mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41435 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:synapsin III KO

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Syn3tm1Pggd

Allele Symbol: Syn3tm1Pggd

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Syn3tm1Pggd/Syn3tm1Pggdinvolves: 129X1/SvJ

cellular phenotype

nervous system phenotype

Genotype: Syn3tm1Pggd/Syn3tm1PggdB6.129X1-Syn3

behavior/neurological phenotype

mammalian phenotype

References

Additional - Syn3tm1Pggd related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Syn3^tm1Pggd

Allele Symbol: Syn3^tm1Pggd

Genotype: Syn3^tm1Pggd/Syn3^tm1Pggd
involves: 129X1/SvJ

Genotype: Syn3^tm1Pggd/Syn3^tm1Pggd
B6.129X1-Syn3

Additional - Syn3^tm1Pggd related