Homozygous synapsin III knockout mice exhibit altered synaptic vesicle trafficking, reduced synaptic depression and delayed axon outgrowth. These mice may be useful in studying the role of synapsin III in neurodevelopment.
Hung-Teh Kao, Nathan Kline Institute
Paul Greengard, Rockefeller University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Syn3 | synapsin III |
The Syn3 gene is a member of the synapsin family of neuronal phosphoproteins, which are associated with neurotransmitter release and neurite outgrowth. Synapsin proteins are associated with autism. Synapsin III localizes to cell bodies, growth cones and presynaptic sites, in contrast to synapsin I and II, which localize only to presynaptic terminals.
The synapsin III knockout allele has a neo cassette replacing exon 1 of the Syn3 gene.
Homozygous mice exhibit an increase in the recycling pool of synaptic vesicles, a reduction in the amplitude of inhibitory postsynaptic currents (IPSC) and a delay in early axon extension.
Homozygous mice are viable and fertile with no reported reproductive deficiency.
A targeting vector was designed by Dr. Paul Greengard (Rockefeller University) to
replace exon 1 of the synapsin 3 gene (Syn3) on chromosome 10 with a neomycin cassette in reverse orientation. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony. The resulting mice were bred with C57BL/6 wildtype mice for at least ten generations.
Allele Name | targeted mutation 1, Paul Greengard |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Syn3tm1Jfe; synapsin III - |
Gene Symbol and Name | Syn3, synapsin III |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 10 |
Molecular Note | A neomycin selection cassette replaced exon 1. Western blot analysis on brain extracts derived from homozygous mice confirmed that no detectable protein was made from this allele. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the synapsin III KO mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #41435 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.