T cells from these TCR transgenic mice recognize intestinal segmented filamentous bacteria (SFB) and predominantly become TH17 cells in SFB-colonized mice. These mice, derived from clone 1A2, are useful in studies of the relationship between commensal microbiota and organ-specific autoimmunity.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
T-helper-17 (TH17) cells play a critical role in mucosal defense and autoimmune disease pathogenesis. They are most abundant in the small intestine lamina propria, where their presence requires colonization of mice with microbiota. Segmented filamentous bacteria (SFB) are sufficient to induce TH17 cells and to promote TH17-dependent autoimmune disease in animal models.
These TCR transgenic mice (derived from clone 1A2) were created to investigate the mechanisms by which distinct bacteria induce the specificity of TH17 cells and foster tissue-specific inflammation. Animals express Vα15 /Vβ14 CDR3 sequences which recognize SFB epitope QFSGAVPNK under the control of the natural TCRα and -β promoter/enhancer elements. SFB-specific T cells become TH17 cells in SFB-colonized mice. Over 90% CD4+ T cells from the peripheral blood are TCRVβ14+ by FACS analysis (as compared to a typical less than 10% for control mice).
Clone 1A2 was derived from a screen for TCR hybridomas prepared from TH17 intestinal CD4+ T cells responding to fecal material from segmented filamentous bacteria (SFB)-monoassociated mice. The receptor recognizes SFB epitope QFSGAVPNK. CDR3 Vβ14 (AWSQGGREEQY) and Vα15 (ATVFMNYNQGKLI) TCR sequences associated with the clone were introduced to vectors that enable transcription under the control of the natural TCRα and -β promoter/enhancer elements. Animals were created using C57BL/6J embryos and maintained on a C57BL/6J genetic background.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 3, Dan R Littman |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | 1A2Tg |
Gene Symbol and Name | Tg(Tcra,Tcrb)3Litt, transgene insertion 3, Dan R Littman |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | Clone 1A2 was derived from a screen for TCR hybridomas prepared from TH17 intestinal CD4+ T cells responding to fecal material from segmented filamentous bacteria (SFB)-monoassociated mice. The receptor recognizes SFB epitope QFSGAVPNK. CDR3 Vbeta14 (AWSQGGREEQY) and Valpha15 (ATVFMNYNQGKLI) TCR sequences associated with the clone were introduced to vectors that enable transcription under the control of the natural TCRalpha and -beta promoter/enhancer elements. |
Hemizygotes are viable and fertile.
When using the 1A2Tg mouse strain in a publication, please cite the originating article(s) and include JAX stock #028204 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non Carrier for Tg(Tcra,Tcrb)3Litt |
Frozen Mouse Embryo | C57BL/6J-Tg(Tcra Tcrb)3Litt/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Tcra Tcrb)3Litt/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Tcra Tcrb)3Litt/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Tg(Tcra Tcrb)3Litt/J Frozen Embryo | $3373.50 |
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