C57BL/6J-Tg(Tcra,Tcrb)3Litt/J

Stock number: 028204
Product name: 1A2Tg
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

T cells from these TCR transgenic mice recognize intestinal segmented filamentous bacteria (SFB) and predominantly become T_H17 cells in SFB-colonized mice. These mice, derived from clone 1A2, are useful in studies of the relationship between commensal microbiota and organ-specific autoimmunity.

Detailed description

T-helper-17 (T_H17) cells play a critical role in mucosal defense and autoimmune disease pathogenesis. They are most abundant in the small intestine lamina propria, where their presence requires colonization of mice with microbiota. Segmented filamentous bacteria (SFB) are sufficient to induce T_H17 cells and to promote TH17-dependent autoimmune disease in animal models.

These TCR transgenic mice (derived from clone 1A2) were created to investigate the mechanisms by which distinct bacteria induce the specificity of T_H17 cells and foster tissue-specific inflammation. Animals express Vα15 /Vβ14 CDR3 sequences which recognize SFB epitope QFSGAVPNK under the control of the natural TCRα and -β promoter/enhancer elements. SFB-specific T cells become T_H17 cells in SFB-colonized mice. Over 90% CD4⁺ T cells from the peripheral blood are TCRVβ14⁺ by FACS analysis (as compared to a typical less than 10% for control mice).

Development

Clone 1A2 was derived from a screen for TCR hybridomas prepared from T_H17 intestinal CD4⁺ T cells responding to fecal material from segmented filamentous bacteria (SFB)-monoassociated mice. The receptor recognizes SFB epitope QFSGAVPNK. CDR3 Vβ14 (AWSQGGREEQY) and Vα15 (ATVFMNYNQGKLI) TCR sequences associated with the clone were introduced to vectors that enable transcription under the control of the natural TCRα and -β promoter/enhancer elements. Animals were created using C57BL/6J embryos and maintained on a C57BL/6J genetic background.

Allele

Symbol: Tg(Tcra,Tcrb)3Litt
Name: transgene insertion 3, Dan R Littman
MGI accession ID: MGI:5661222
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Tcra,Tcrb)3Litt
Marker name: transgene insertion 3, Dan R Littman
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: Clone 1A2 was derived from a screen for TCR hybridomas prepared from TH17 intestinal CD4+ T cells responding to fecal material from segmented filamentous bacteria (SFB)-monoassociated mice. The receptor recognizes SFB epitope QFSGAVPNK. CDR3 Vbeta14 (AWSQGGREEQY) and Valpha15 (ATVFMNYNQGKLI) TCR sequences associated with the clone were introduced to vectors that enable transcription under the control of the natural TCRalpha and -beta promoter/enhancer elements.