This allele replaces the coding sequence of the G-protein coupled olfactory receptor Olfr151 with the coding sequence of Mc4r, a G-protein coupled receptor not usually expressed in the main olfactory epithelium. MC4R binds GNAS and shares 14% amino acid identity with OLFR151. This substitution allele is useful for assessing the minimal sequence requirements for a G protein coupled receptor that is driven by the promoter of an olfactory sensory receptor to facilitate monoallelic expression of olfactory receptors, coalescence of olfactory glomeruli, and signal transduction via cAMP in olfactory sensory neurons.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Inserted expressed sequence) | Olfr151 | olfactory receptor 151 |
This substitution allele is partially successful as a surrogate olfactory receptor, but not as good as the substitution of beta 2 adrenergic receptor (see Stock# 006734). Consistent with normal allelic exclusion of olfactory receptors, olfactory sensory neurons that express this GFP tagged MC4R substitution allele do not express any other olfactory receptor, proving this sequence adequate for monoallelic expression. However, the level of expression and number of olfactory neurons expressing this substitution allele are reduced compared with that of endogenous OLFR151 on non-mutant olfactory sensory neurons. Subcellular localization of this MC4R on olfactory sensory neurons is highly concentrated in the dendritic knob and endings of the cilia. Patch-clamp recordings show that these neurons respond to the MC4R selective agonist Ro27-3225. The axons from these neurons coalesce into smaller than normal glomeruli that are positioned in a more anterior position than the endogenous OLFR151 glomeruli normally are.
The coding sequence of Olfr151 was replaced by the sequence encoding
amino acids 1-333 of Mc4r and an IRES-tauGFP. The targeted mutation was
generated in 129P2/OlaHsd-derived E14 ES cells. The ACNf neomycin
cassette was self-excised by Cre recombination in the germline of the
male chimeras, which were then bred with C57BL6/J females. After several generations of sibling breeding sperm was cryopreserved from homozygous males.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Mc4r, melanocortin 4 receptor, mouse, laboratory |
Site of Expression | Olfactory sensory neurons |
Allele Name | targeted mutation 42, Peter Mombaerts |
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Allele Type | Targeted (Reporter, Inserted expressed sequence) |
Allele Synonym(s) | Mc4r-M71-IRES-tauGFP |
Gene Symbol and Name | Olfr151, olfactory receptor 151 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Mc4r, melanocortin 4 receptor, mouse, laboratory |
Site of Expression | Olfactory sensory neurons |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | The endogenous coding sequence was replaced by the sequence encoding amoino acids 1 through 333 of Mc4r followed by an IRES-tauGFP cassette for bicistronic expression. The ACNf neomycin selection cassette was self-excised by Cre recombination in the germline of the male chimera. |
Homozygotes are viable and fertile. The cryopreserved sperm were from homozygous males so recovered pups will be heterozygous.
When using the B6;129P2-Olfr151tm42(Mc4r)Mom/MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #028199 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Olfr151<tm42(Mc4r)Mom> |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm42(Mc4r)Mom>/MomJ Mc4r->M71-IRES-tauGFP | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm42(Mc4r)Mom>/MomJ Mc4r->M71-IRES-tauGFP | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm42(Mc4r)Mom>/MomJ Mc4r->M71-IRES-tauGFP | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm42(Mc4r)Mom>/MomJ Mc4r->M71-IRES-tauGFP | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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