These Dbx1CreERT2 mice have Cre-ERT2 knocked into the 3’ UTR region of the developing brain homeobox 1 gene (Dbx1). This strain may be useful for mediating inducible cre recombination in Dbx1-expressing tissues, as well as for CNS neuronal progenitor subpopulation fate mapping.
Joshua Corbin, Children's National Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing, Inducible) | Dbx1 | developing brain homeobox 1 |
A targeting vector containing an IRES-CreERT2 cassette (IRES, CreERT2 (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), FRTsite flanked NEO, and PolyA signal) was inserted into the 3' UTR region of exon 4 of the Dbx1 gene.
This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+
derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were bred with 129S9/SvEvH (129/SvEv) mice. Mutant mice were then crossed to ROSA26::FLPe knock in mice on the 129S4/SvJaeSor genetic background; (Stock No. 003946) to remove the neo selection cassette.
The donating investigator reported that the mice were then backcrossed to 129S9/SvEvH for approximately 15 generations (see SNP note below). Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize 129S1/SvImJ oocytes (Stock No. 002448).
A 33 SNP (single nucleotide polymorphism) panel analysis, with 28 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Seven of the markers (on chromosome 1, 2, 6, 8, 16, and X) were found to be segregating with a C57BL/6 genetic background. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed 129;B6 genetic background.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | Preoptic area neurons. |
Allele Name | targeted mutation 1, Joshua Corbin |
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Allele Type | Targeted (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Dbx1CreERT2 |
Gene Symbol and Name | Dbx1, developing brain homeobox 1 |
Gene Synonym(s) | |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | Preoptic area neurons. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | An IRES-cre/ERT2 (Esr1), and neo cassette were inserted into the downstream untranslated region. Germ line flp mediated recombination was used to remove the neo cassette. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Dbx1CreERT2 Knockin mouse strain in a publication, please cite the originating article(s) and include JAX stock #028131 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Dbx1<tm1(cre/ERT2)Jcor> |
Frozen Mouse Embryo | 129;B6-Dbx1<tm1(cre/ERT2)Jcor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129;B6-Dbx1<tm1(cre/ERT2)Jcor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129;B6-Dbx1<tm1(cre/ERT2)Jcor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | 129;B6-Dbx1<tm1(cre/ERT2)Jcor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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