B6;CBA-Tg(Trp53)1Srn/J

Stock number: 028130
Product name: Super p53
Research areas: Cancer Research, Cell Biology Research, Research Tools

Description

Super p53 mice overexpress Trp53, leading to enhanced DNA damage response and resistance to tumors.

Detailed description

Mice homozygous for the BAC Super p53 transgene are viable and fertile. The donating investigator reports that the BAC transgene inserted as a single copy in the genome. Therefore, homozygous mice express two extra copies of the wild-type mouse Trp53 (transformation related protein 53) gene, one on each homologous chromosome, directed by its endogenous promoter/enhancer regions on the BAC transgene. Trp53 encodes a tumor suppressor protein, which also functions as a transcription factor. It can trigger proliferation arrest and cell death in response to stresses such as DNA damage and oncogenic stress. Hemizygous and Homozygous Super p53 mice show an dose-dependant enhanced response to DNA damage and they are protected from cancer. They also age normally in comparison to other p53 over expressing mouse lines.

Development

Bacterial artificial chromosome (BAC) library was used to obtain a 130 kb BAC containing the entire mouse Trp53 (transformation related protein 53) gene. This BAC also contains other active genes such as Efnb3 (Ephrin B3). The BAC was microinjected into fertilized (C57BL/6 x CBA)F2 oocytes. Super p53 mice, carrying one copy of the BAC, were maintained by breeding transgenic mice with C57BL/6JOlaHsd inbred mice for at least 10 generations by the donating lab (see SNP note below). Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Four of the 27 markers throughout the genome were segregating, suggesting an incomplete backcross.

Allele

Symbol: Tg(Trp53)1Srn
Name: transgene insertion 1, Manuel Serrano
MGI accession ID: MGI:4360913
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: p53-tg; super p53
Marker symbol: Tg(Trp53)1Srn
Marker name: transgene insertion 1, Manuel Serrano
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F2
Expressed genes: Trp53,transformation related protein 53,mouse, laboratory
Molecular note: Bacterial artificial chromosome (BAC) library was used to obtain a 130 kb BAC containing the entire mouse Trp53 (transformation related protein 53) gene in it's "natural genomic context". Trp53 is located at the terminal end of the segment. The other end of the BAC falls in the middle of the Dnahc2 gene. This line carries one copy of the transgene.