Bacterial artificial chromosome (BAC) library was used to obtain a 130 kb BAC containing the entire mouse Trp53 (transformation related protein 53) gene. This BAC also contains other active genes such as Efnb3 (Ephrin B3). The BAC was microinjected into fertilized (C57BL/6 x CBA)F2 oocytes. Super p53 mice, carrying one copy of the BAC, were maintained by breeding transgenic mice with C57BL/6JOlaHsd inbred mice for at least 10 generations by the donating lab (see SNP note below). Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Four of the 27 markers throughout the genome were segregating, suggesting an incomplete backcross.
