Overview

Also Known As:Wnt10b-G2aCE

The Wnt10b^G2aCE knock-in allele was designed to both abolish Wnt10b gene expression and express EGFP and Cre-ER^T2 proteins from the Wnt10b promoter in adult prostate and bladder.

Donating Investigator

Andrew P McMahon, University of Southern California

Genetic overview

Genetic Background	Generation

Wnt10b^{tm1(cre/ERT2)Amc}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Reporter, Inducible, Null/Knockout, RMCE-ready)	Wnt10b	wingless-type MMTV integration site family, member 10B

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Research Applications

Research Tools
Internal/Organ Research
Developmental Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Wnt10b^G2aCE knock-in allele was designed to both abolish wingless-type MMTV integration site family, member 10B (Wnt10b) gene expression and express individual EGFP and Cre-ER^T2 proteins from the Wnt10b promoter/enhancer elements. WNT10B encodes a signaling protein involved in the regulation of cell fate and patterning during embryogenesis. Heterozygous Wnt10b^G2aCE mice are viable and fertile. The donating investigator has not attempted to make this strain homozygous. Weak EGFP fluorescence is seen in the skin of 5 day old mice. Cre-ER^T2 gene activity is inducible and can be observed following tamoxifen administration. As such, when these mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in prostate, epididymis, bladder and skin. Specifically, when tamoxifen is induced at P3, Cre-mediated recombination occurs in skin and keratin-8 (Krt8)-expressing cells of the prostate starting at P5, and in the epididymis at 12 weeks of age.

This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).

Development

An exchange vector was designed to be inserted downstream of exon 1 of the wingless-type MMTV integration site family, member 10B (Wnt10b) gene using dual-recombinase mediated cassette exchange (dRMCE). Specifically, the exchange vector contained (from 5’ to 3’) a frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) sequence, a viral T2A oligopeptide, and a Cre-ER^T2 sequence (Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain). A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector.

This exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-A^tm1Brd-derived JM8A1.N3 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone EPD0314_2_A09. Recombined ES cells, with the neo cassette removed and resistant to puro, were injected into B6(Cg)-Tyr^c-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. This strain may be segregating for A from the ES cell used in it's

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Cre-ERT2 fusion protein and EGFP are expressed from endogenous Wnt10b promoter/enhancer elements in the prostate, epididymis, bladder and skin.

Control Suggestions

Wild-type from the colony
Noncarrier

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

McMahon A; Zhang P and colleagues; The GenitoUrinary Development Molecular Anatomy Project (GUDMAP). 2010. Summary of mouse strains characterized by GUDMAP consortium. UnpublishedMGI: J:159099

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Genetics

Wnt10b^{tm1(cre/ERT2)Amc}

Allele Symbol: Wnt10b^{tm1(cre/ERT2)Amc}

Allele Name	targeted mutation 1, Andrew P McMahon
Allele Type	Targeted (Recombinase-expressing, Reporter, Inducible, Null/Knockout, RMCE-ready)
Allele Synonym(s)	Wnt10b^{tm1(GFP,cre/ERT2)Amc}; Wnt10b-G2aCE
Gene Symbol and Name	Wnt10b, wingless-type MMTV integration site family, member 10B
Gene Synonym(s)
Promoter	Wnt10b, wingless-type MMTV integration site family, member 10B, mouse, laboratory
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Cre-ERT2 fusion protein and EGFP are expressed from endogenous Wnt10b promoter/enhancer elements in the prostate, epididymis, bladder and skin.
Strain of Origin	C57BL/6N-A^tm1Brd
Chromosome	15
General Note	The exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-derived JM8A1.N3 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone EPD0314_2_A09. Recombined ES cells lack the neo cassette and are resistant to puromycin.
Molecular Note	An exchange vector was designed to be inserted downstream of exon 1 of the gene using dual-recombinase mediated cassette exchange (dRMCE). Specifically, the exchange vector contained (from 5 to 3) an FRT site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein sequence, a viral T2A oligopeptide, and a Cre-ERT2 sequence (Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain). A rox-flanked puromycin resistance cassette and a loxP site were inserted at the 3' end of the exchange vector.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Fluorescent Proteins
- Cre-lox System
  - Cre Recombinase Expression: Inducible
  - Cre Recombinase Expression
- Reproductive Biology Research
  - Cre-lox System
Internal/Organ Research
- Prostate
- Bladder
Developmental Biology Research
- Internal/Organ Defects
  - skin
  - prostate

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Wnt10b^{tm1(cre/ERT2)Amc}/Wnt10b⁺
C57BL/6-Wnt10b

mammalian phenotype

no phenotypic analysis

References

McMahon A; Zhang P and colleagues; The GenitoUrinary Development Molecular Anatomy Project (GUDMAP). 2010. Summary of mouse strains characterized by GUDMAP consortium. UnpublishedMGI: J:159099

Additional - Wnt10b^{tm1(cre/ERT2)Amc} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Wnt10b-Alternate 1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). The donating investigator has not attempted to make this strain homozygous.
- Additional Breeding and Husbandry Support
Mating System
- Wild-type x Heterozygote
- Heterozygote x Wild-type
Citation
When using the Wnt10b-G2aCE mouse strain in a publication, please cite the originating article(s) and include JAX stock #028116 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Wnt10b<tm1(cre/ERT2)Amc>

Related Products and Services

Frozen Mouse Embryo	C57BL/6-Wnt10b<tm1(cre/ERT2)Amc>/J	$2595.00
Frozen Mouse Embryo	C57BL/6-Wnt10b<tm1(cre/ERT2)Amc>/J	$2595.00
Frozen Mouse Embryo	C57BL/6-Wnt10b<tm1(cre/ERT2)Amc>/J	$3373.50
Frozen Mouse Embryo	C57BL/6-Wnt10b<tm1(cre/ERT2)Amc>/J	$3373.50

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Wnt10b-G2aCE

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Wnt10btm1(cre/ERT2)Amc

Allele Symbol: Wnt10btm1(cre/ERT2)Amc

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Wnt10btm1(cre/ERT2)Amc/Wnt10b+C57BL/6-Wnt10b

mammalian phenotype

References

Additional - Wnt10btm1(cre/ERT2)Amc related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Wnt10b^{tm1(cre/ERT2)Amc}

Allele Symbol: Wnt10b^{tm1(cre/ERT2)Amc}

Genotype: Wnt10b^{tm1(cre/ERT2)Amc}/Wnt10b⁺
C57BL/6-Wnt10b

Additional - Wnt10b^{tm1(cre/ERT2)Amc} related