The Wnt10bG2aCE knock-in allele was designed to both abolish wingless-type MMTV integration site family, member 10B (Wnt10b) gene expression and express individual EGFP and Cre-ERT2 proteins from the Wnt10b promoter/enhancer elements. WNT10B encodes a signaling protein involved in the regulation of cell fate and patterning during embryogenesis. Heterozygous Wnt10bG2aCE mice are viable and fertile. The donating investigator has not attempted to make this strain homozygous. Weak EGFP fluorescence is seen in the skin of 5 day old mice. Cre-ERT2 gene activity is inducible and can be observed following tamoxifen administration. As such, when these mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in prostate, epididymis, bladder and skin. Specifically, when tamoxifen is induced at P3, Cre-mediated recombination occurs in skin and keratin-8 (Krt8)-expressing cells of the prostate starting at P5, and in the epididymis at 12 weeks of age. 
 
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).

The Wnt10bG2aCE knock-in allele was designed to both abolish Wnt10b gene expression and express EGFP and Cre-ERT2 proteins from the Wnt10b promoter in adult prostate and bladder.

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Our minimum guarantee for cryorecovery orders is 2 carriers. To ensure that your colony can be rapidly expanded upon arrival, additional carriers may be added to your order.