| Allele Name | targeted mutation 1, Andrew P McMahon |
| Allele Type | Targeted (Recombinase-expressing, Reporter, Inducible, Null/Knockout, RMCE-ready) |
| Allele Synonym(s) | Wnt10btm1(GFP,cre/ERT2)Amc; Wnt10b-G2aCE |
| Gene Symbol and Name | Wnt10b, wingless-type MMTV integration site family, member 10B |
| Gene Synonym(s) | |
| Promoter | Wnt10b, wingless-type MMTV integration site family, member 10B, mouse, laboratory |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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| Site of Expression | Cre-ERT2 fusion protein and EGFP are expressed from endogenous Wnt10b promoter/enhancer elements in the prostate, epididymis, bladder and skin. |
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| Strain of Origin | C57BL/6N-Atm1Brd |
| Chromosome | 15 |
| General Note | The exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-derived JM8A1.N3 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone EPD0314_2_A09. Recombined ES cells lack the neo cassette and are resistant to puromycin. |
| Molecular Note | An exchange vector was designed to be inserted downstream of exon 1 of the gene using dual-recombinase mediated cassette exchange (dRMCE). Specifically, the exchange vector contained (from 5 to 3) an FRT site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein sequence, a viral T2A oligopeptide, and a Cre-ERT2 sequence (Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain). A rox-flanked puromycin resistance cassette and a loxP site were inserted at the 3' end of the exchange vector. |
