STOCK Gata3^tm1.1Mbu/J

Stock number: 028103
Product name: Gata3^fl
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 4 of the Gata3 gene. This strain may be useful for generating conditional mutations in applications related to T cell development, kidney development and prostate cancer progression.

Detailed description

The Gata3 gene encodes a zinc-finger transcription factor important during T cell development, kidney development, in long-term hematopoietic stem cells self-renewal and prostate cancer progression. Mutations in the human GATA3 gene are associated with hypoparathyroidism, sensorineural deafness, and renal dysplasia syndrome. These mice possess loxP sites on either side of exon 4 of the targeted Gata3 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele.

When bred to a strain with Cre recombinase expression in CD4-expressing T cells (see Stock No. 022071 for example), this mutant mouse strain may be useful in studies of T cell proliferation and homeostasis.

Development

A targeting vector consisting of FRT sites flanking a STOP cassette, IRES-GFP, poly adenylation sequence and NEO selection cassette, was utilized in the construction of this mutant. This selection cassette and a 3’ loxP site were inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129P2/OlaHsd-Hprt^b-m3 derived HM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The Gata3^ex4GFP/+ mice were then crossed to transgenic mice, on an unknown genetic background, expressing FLPe recombinase (Stock No. 005703, for example), producing progeny carrying the Gata3^F (Gata3^fl), with exon 4 floxed. The donating investigator reported that these mice were then backcrossed to C57BL/6J for more than 10 generations (see SNP note below). Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Two of the 27 markers throughout the genome were segregating, suggesting an incomplete backcross. Also, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Gata3^tm1.1Mbu
Name: targeted mutation 1.1, Meinrad Busslinger
MGI accession ID: MGI:3719568
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Gata3
Marker name: GATA binding protein 3
Chromosome: 2
Strain of origin: 129P2/OlaHsd-Hprt1^b-m3
Molecular note: Expression of FRT recombinase resulted in the removal of the stop-IRES-GFP-neo cassette.