These floxed mutant mice possess loxP sites flanking exon 4 of the Gata3 gene. This strain may be useful for generating conditional mutations in applications related to T cell development, kidney development and prostate cancer progression.
Meinrad Busslinger, Research Institute of Molecular Pathology (IMP)
The Gata3 gene encodes a zinc-finger transcription factor important during T cell development, kidney development, in long-term hematopoietic stem cells self-renewal and prostate cancer progression. Mutations in the human GATA3 gene are associated with hypoparathyroidism, sensorineural deafness, and renal dysplasia syndrome.
These mice possess loxP sites on either side of exon 4 of the targeted Gata3 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele.
When bred to a strain with Cre recombinase expression in CD4-expressing T cells (see Stock No. 022071 for example), this mutant mouse strain may be useful in studies of T cell proliferation and homeostasis.
A targeting vector consisting of FRT sites flanking a STOP cassette, IRES-GFP, poly adenylation sequence and NEO selection cassette, was utilized in the construction of this mutant. This selection cassette and a 3’ loxP site were inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129P2/OlaHsd-Hprtb-m3 derived HM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The Gata3ex4GFP/+ mice were then crossed to transgenic mice, on an unknown genetic background, expressing FLPe recombinase (Stock No. 005703, for example), producing progeny carrying the Gata3F (Gata3fl), with exon 4 floxed. The donating investigator reported that these mice were then backcrossed to C57BL/6J for more than 10 generations (see SNP note below).
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Two of the 27 markers throughout the genome were segregating, suggesting an incomplete backcross. Also, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1.1, Meinrad Busslinger|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Gata3tm1.1Mbu; targeted mutation 1.1, Meinrad Busslinger|
|Gene Symbol and Name||Gata3, GATA binding protein 3|
|Gene Synonym(s)||HDR; juvenile alopecia; HDRS; jal; jal; Gata-3; Gata-3|
|Strain of Origin||129P2/OlaHsd-Hprtb-m3|
|Molecular Note||Expression of FRT recombinase resulted in the removal of the stop-IRES-GFP-neo cassette.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Gata3fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #028103 in your Materials and Methods section.
|Heterozygous for Gata3<tm1.1Mbu>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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