These Gata6H2B-Venus knock-in reporter mice are suitable for use in applications related to live imaging and isolation of cells expressing Gata6, and the study of endoderm and mesoderm development.
Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout) | Gata6 | GATA binding protein 6 |
The Gata6 gene encodes a zinc finger transcription factor that is important in heart and endoderm development. These knock-in/knock-out mice express Venus variant Yellow Fluorescent Protein from the endogenous Gata6 locus.
Venus is detected in mesoderm and endoderm progenitor cells after endoderm differentiation in heterozygous embryos. Venus fluorescence is similar to endogenous Gata6 expression patterns. In heterozygous E8.25 embryos fluorescence is not detected in lateral mesoderm derived endocardium and body wall, but is detected in the yolk sac. In adult heterozygotes, fluorescence is detected in heart, lung, pancreas, liver, gall bladder, ovaries, adrenal glands, stomach (mucosa and mesothelium of the gastric corpus and small intestine), bladder, and skin (sebaceous glands).
Mice that are heterozygous for the targeted mutation are viable, and may have reduced fertility (the number of adult heterozygotes are lower than the expected Mendelian ratio). Homozygotes are not viable. No Gata6 gene product (protein) is detected by immunostaining/immunofluorescence of homozygous E3.5 embryos.
A targeting vector designed by Dr. Anna-Katerina Hadjantonakis (Memorial Sloan Kettering Cancer Center) containing (3' to 5'): FRT site, engrailed 2 intron and exon with splice acceptor site, H2B-Venus-polyadenylation sequence, rox site flanked beta actin promoter driven NEO cassette, loxP site flanked Gata6 exon 2, was used to disrupt intron 1 of the Gata6 gene, upstream of the translation initiation codons.
The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1 embryonic stem (ES) cells derived from KH2 ES cell mice carrying Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae alleles. (The KH2 ES cell mice were originally generated in (C57BL/6 x 129S4/SvJae)F1 derived v6.5 ES cells) Correctly targeted ES cells were injected into C2J blastocysts. The resulting chimeric males were crossed to CD-1 female mice. The Gata6tm1Hadj (Gata6H2B-Venus) allele was bred away from the other alleles through breeding to CD-1 females.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Site of Expression | Venus variant Yellow Fluorescent Protein is detected in mesoderm and endoderm progenitor cells. |
Allele Name | targeted mutation 1, Anna-Katerina Hadjantonakis |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout) |
Allele Synonym(s) | Gata6H2B-Venus; Gata6tm1(HIST1H2BB/Venus)Hadj |
Gene Symbol and Name | Gata6, GATA binding protein 6 |
Gene Synonym(s) | |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | Venus variant Yellow Fluorescent Protein is detected in mesoderm and endoderm progenitor cells. |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 18 |
General Note | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm2(tetO-Pou5f1)Jae (J:159351). |
Molecular Note | The targeting vector is designed to insert downstream of the first non-coding intron with (from 3' to 5') an FRT site, engrailed 2 intron and exon with splice acceptor site, H2B-Venus-polyadenylation sequence, rox-flanked beta actin promoter driven NEO cassette, and a loxP site, followed by exon 2 and a second loxP site. Two alternative translation initiation codons are present in exon 2. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes are not viable and heterozygotes may have reduced fertility (heterozygous adult mice occur at less than expected Mendelian ratio).
When using the Gata6H2B-Venus knock-in reporter mouse strain in a publication, please cite the originating article(s) and include JAX stock #028096 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gata6<tm1Hadj> |
Frozen Mouse Embryo | STOCK Gata6<tm1Hadj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gata6<tm1Hadj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gata6<tm1Hadj>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Gata6<tm1Hadj>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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