Overview

Also Known As: Gata6H2B-Venus knock-in reporter

These Gata6^H2B-Venus knock-in reporter mice are suitable for use in applications related to live imaging and isolation of cells expressing Gata6, and the study of endoderm and mesoderm development.

Donating Investigator

Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center

Genetic overview

Genetic Background	Generation

Gata6^tm1Hadj

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout)	Gata6	GATA binding protein 6

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Research Applications

Research Tools
Developmental Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Gata6 gene encodes a zinc finger transcription factor that is important in heart and endoderm development. These knock-in/knock-out mice express Venus variant Yellow Fluorescent Protein from the endogenous Gata6 locus.
Venus is detected in mesoderm and endoderm progenitor cells after endoderm differentiation in heterozygous embryos. Venus fluorescence is similar to endogenous Gata6 expression patterns. In heterozygous E8.25 embryos fluorescence is not detected in lateral mesoderm derived endocardium and body wall, but is detected in the yolk sac. In adult heterozygotes, fluorescence is detected in heart, lung, pancreas, liver, gall bladder, ovaries, adrenal glands, stomach (mucosa and mesothelium of the gastric corpus and small intestine), bladder, and skin (sebaceous glands).
Mice that are heterozygous for the targeted mutation are viable, and may have reduced fertility (the number of adult heterozygotes are lower than the expected Mendelian ratio). Homozygotes are not viable. No Gata6 gene product (protein) is detected by immunostaining/immunofluorescence of homozygous E3.5 embryos.

Development

A targeting vector designed by Dr. Anna-Katerina Hadjantonakis (Memorial Sloan Kettering Cancer Center) containing (3' to 5'): FRT site, engrailed 2 intron and exon with splice acceptor site, H2B-Venus-polyadenylation sequence, rox site flanked beta actin promoter driven NEO cassette, loxP site flanked Gata6 exon 2, was used to disrupt intron 1 of the Gata6 gene, upstream of the translation initiation codons.
The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1 embryonic stem (ES) cells derived from KH2 ES cell mice carrying Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} alleles. (The KH2 ES cell mice were originally generated in (C57BL/6 x 129S4/SvJae)F1 derived v6.5 ES cells) Correctly targeted ES cells were injected into C2J blastocysts. The resulting chimeric males were crossed to CD-1 female mice. The Gata6^tm1Hadj (Gata6^H2B-Venus) allele was bred away from the other alleles through breeding to CD-1 females.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Expression Data

Expressed Gene	YFP, Yellow Fluorescent Protein, jellyfish
Site of Expression	Venus variant Yellow Fluorescent Protein is detected in mesoderm and endoderm progenitor cells.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Freyer L; Schroter C; Saiz N; Schrode N; Nowotschin S; Martinez-Arias A; Hadjantonakis AK. 2015. A loss-of-function and H2B-Venus transcriptional reporter allele for Gata6 in mice. BMC Dev Biol 15:38PubMed: 26498761 MGI: J:226600

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Genetics

Gata6^tm1Hadj

Allele Symbol: Gata6^tm1Hadj

Allele Name	targeted mutation 1, Anna-Katerina Hadjantonakis
Allele Type	Targeted (Conditional ready (e.g. floxed), Reporter, Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Anna-Katerina Hadjantonakis; Gata6^tm1Hadj
Gene Symbol and Name	Gata6, GATA binding protein 6
Gene Synonym(s)	expressed sequence AA410133; AA410133
Expressed Gene	YFP, Yellow Fluorescent Protein, jellyfish
Site of Expression	Venus variant Yellow Fluorescent Protein is detected in mesoderm and endoderm progenitor cells.
Strain of Origin	(C57BL/6 x 129S4/SvJae)F1
Chromosome	18
General Note	The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTAM2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae} (J:159351*).
Molecular Note	The targeting vector is designed to insert downstream of the first non-coding intron with (from 3' to 5') an FRT site, engrailed 2 intron and exon with splice acceptor site, H2B-Venus-polyadenylation sequence, rox-flanked beta actin promoter driven NEO cassette, and a loxP site, followed by exon 2 and a second loxP site. Two alternative translation initiation codons are present in exon 2.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Developmental Biology Research
- Fluorescent Proteins
Developmental Biology Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gata6^tm1Hadj/Gata6⁺
involves: 129S4/SvJae * C57BL/6 * CD-1

mortality/aging

prenatal lethality, incomplete penetrance
- heterozygote x wild-type matings produce fewer heterozygotes than the expected Mendelian ratios

reproductive system phenotype

reduced fertility

References

Freyer L; Schroter C; Saiz N; Schrode N; Nowotschin S; Martinez-Arias A; Hadjantonakis AK. 2015. A loss-of-function and H2B-Venus transcriptional reporter allele for Gata6 in mice. BMC Dev Biol 15:38PubMed: 26498761 MGI: J:226600

Additional - Gata6^tm1Hadj related

Technical Support

Contact Technical Support

Genotyping Protocols
- SEPARATED MELT: Gata6^tm1Hadj Alternate1
- MELT: Generic Neo
- Probe: Gata6^tm1Hadj Probe
- Probe: Generic Neo
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes are not viable and heterozygotes may have reduced fertility (heterozygous adult mice occur at less than expected Mendelian ratio).
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x Wild-type
- Wild-type x Heterozygote
Citation
When using the Gata6H2B-Venus knock-in reporter mouse strain in a publication, please cite the originating article(s) and include JAX stock #028096 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Gata6<tm1Hadj>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Licensing Information

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Also Known As: Gata6H2B-Venus knock-in reporter

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Gata6tm1Hadj

Allele Symbol: Gata6tm1Hadj

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gata6tm1Hadj/Gata6+involves: 129S4/SvJae * C57BL/6 * CD-1

mortality/aging

reproductive system phenotype

References

Additional - Gata6tm1Hadj related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Gata6^tm1Hadj

Allele Symbol: Gata6^tm1Hadj

Genotype: Gata6^tm1Hadj/Gata6⁺
involves: 129S4/SvJae * C57BL/6 * CD-1

Additional - Gata6^tm1Hadj related