B6.129S-Gipc1^tm1.1Mhsi/J

Stock number: 028090
Product name: Gipc1^flox
Research areas: Cardiovascular Research, Research Tools

Description

Gipc1^flox/flox mice possess loxP sites flanking exon 2 of the Gipc1 gene. This strain may be useful for studying the regulation of arteriogenesis.

Detailed description

Gipc1^flox/flox mice possess loxP sites flanking exon 2 of the GIPC PDZ domain containing family, member 1 Gipc1 gene. Gipc1, also known as Synectin, is a PDZ scaffold protein shown to be a key regulator in the formation of arterial vasculature. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Tek-cre)12Flv/J mice (Stock No. 004128) expressing Cre Recombinase in endothelial cells, resulting offspring exhibit less branching of arterial trees in both the heart and kidneys, which appear smaller in diameter, and they have fewer artery-to-artery connections than those seen in littermate controls. They also have a decrease in the number of smaller arteries, and exhibit aneurysmal dilations of distal coronary arteries and tubular aneurysms.

Development

A targeting vector was designed by Dr. Michael Simons (Yale University) to insert a loxP site upstream of exon 2 and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 2 of the GIPC PDZ domain containing family, member 1 (Gipc1) gene. The construct was electroporated into 129SvIMJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice. Offspring were bred with B6.129S4-Gt(ROSA)26Sor^tm1(FLP1)Dym/RainJ mice (Stock No. 009086) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Resulting Gipc1^flox/flox mice were bred to C57BL/6J mice for at least seven generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Gipc1^tm1.1Mhsi
Name: targeted mutation 1.1, Michael Simons
MGI accession ID: MGI:5644333
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Gipc1
Marker name: GIPC PDZ domain containing family, member 1
Chromosome: 8
Strain of origin: 129S1/SvImJ
Molecular note: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed.