Gipc1flox/flox mice possess loxP sites flanking exon 2 of the Gipc1 gene. This strain may be useful for studying the regulation of arteriogenesis.
Michael Simons, Yale University
Gipc1flox/flox mice possess loxP sites flanking exon 2 of the GIPC PDZ domain containing family, member 1 Gipc1 gene. Gipc1, also known as Synectin, is a PDZ scaffold protein shown to be a key regulator in the formation of arterial vasculature. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
For example, when bred to B6.Cg-Tg(Tek-cre)12Flv/J mice (Stock No. 004128) expressing Cre Recombinase in endothelial cells, resulting offspring exhibit less branching of arterial trees in both the heart and kidneys, which appear smaller in diameter, and they have fewer artery-to-artery connections than those seen in littermate controls. They also have a decrease in the number of smaller arteries, and exhibit aneurysmal dilations of distal coronary arteries and tubular aneurysms.
A targeting vector was designed by Dr. Michael Simons (Yale University) to insert a loxP site upstream of exon 2 and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 2 of the GIPC PDZ domain containing family, member 1 (Gipc1) gene. The construct was electroporated into 129SvIMJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice. Offspring were bred with B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ mice (Stock No. 009086) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Resulting Gipc1flox/flox mice were bred to C57BL/6J mice for at least seven generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, Michael Simons|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||targeted mutation 1.1, Michael Simons; Gipc1tm1.1Mhsi|
|Gene Symbol and Name||Gipc1, GIPC PDZ domain containing family, member 1|
|Gene Synonym(s)||GLUT1CBP; neurophilin1-IP; C19orf3; Rgs19ip1; NIP; semaF cytoplasmic domain associated protein 1; SYNECTIIN; SYNECTIN; TIP-2; Hs.6454; IIP-1; Semcap1; Rgs19; RGS19IP1; SEMCAP; Semcap1; GIPC; regulator of G-protein signaling 19 interacting protein 1; Glut1CIP; TaxIP2; Rgs19ip1; synectin; Gipc|
|Strain of Origin||129S1/SvImJ|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Gipc1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #028090 in your Materials and Methods section.
|Heterozygous or wildtype for Gipc1<tm1.1Mhsi>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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