Gipc1flox/flox mice possess loxP sites flanking exon 2 of the Gipc1 gene. This strain may be useful for studying the regulation of arteriogenesis.
Michael Simons, Yale University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Gipc1 | GIPC PDZ domain containing family, member 1 |
Gipc1flox/flox mice possess loxP sites flanking exon 2 of the GIPC PDZ domain containing family, member 1 Gipc1 gene. Gipc1, also known as Synectin, is a PDZ scaffold protein shown to be a key regulator in the formation of arterial vasculature. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
For example, when bred to B6.Cg-Tg(Tek-cre)12Flv/J mice (Stock No. 004128) expressing Cre Recombinase in endothelial cells, resulting offspring exhibit less branching of arterial trees in both the heart and kidneys, which appear smaller in diameter, and they have fewer artery-to-artery connections than those seen in littermate controls. They also have a decrease in the number of smaller arteries, and exhibit aneurysmal dilations of distal coronary arteries and tubular aneurysms.
A targeting vector was designed by Dr. Michael Simons (Yale University) to insert a loxP site upstream of exon 2 and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 2 of the GIPC PDZ domain containing family, member 1 (Gipc1) gene. The construct was electroporated into 129SvIMJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice. Offspring were bred with B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ mice (Stock No. 009086) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Resulting Gipc1flox/flox mice were bred to C57BL/6J mice for at least seven generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1.1, Michael Simons |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | gipc1flox |
Gene Symbol and Name | Gipc1, GIPC PDZ domain containing family, member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S1/SvImJ |
Chromosome | 8 |
Molecular Note | A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Gipc1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #028090 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gipc1<tm1.1Mhsi> |
Frozen Mouse Embryo | B6.129S-Gipc1<tm1.1Mhsi>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Gipc1<tm1.1Mhsi>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Gipc1<tm1.1Mhsi>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S-Gipc1<tm1.1Mhsi>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.