ABH/BiozDbakJ[cc]

Stock number: 027904
Product name: Biozzi
Strain synonyms: ABH/QMUL

Description

These inbred mice carry the H2 ^dq1 MHC haplotype as well as the mouse I-A^g7 MHC class II molecule found in NOD inbred mice. This strain is used for studying the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS) and is susceptible to induced autoimmunities.

Detailed description

The ABH (AB/H, antibody high) inbred strain was derived from outbred Swiss albino mice selected by Dr. Guido Biozzi for high antibody response to sheep red blood cells (Feingold, 1976). ABH animals carry a unique MHC haplotype (H2^dq1) and express the mouse I-A^g7 MHC class II molecule found in NOD inbred mice. Animals are not diabetic.

ABH mice receiving subcutaneous injections of adjuvanted spinal cord homogenate develop experimental autoimmune encephalomyelitis (EAE). Animals are reported to typically develop hind limb paralysis 15-20 days post-inoculation, preceded by a progressive weight loss. Symptoms in the majority of animals go into remission with some lingering neurological deficit. Relapsing episodes are characterized by an increase in the severity of clinical signs. Both males and females are susceptible. A full working protocol may be found in Mult Scler Relat Disord. 2012 Jan;1(1):29-38).

For more details, please see the Mouse Genome Informatics entry for ABH inbred mice.

Development

This inbred strain was derived from outbred Swiss albino mice selected by Dr. Guido Biozzi (Pasteur Insitute) for high antibody response to sheep red blood cells (Feingold, 1976).

Animals were obtained by the Royal College of Surgeons in England in about 1986 and maintained by Dr. David Baker for more than 40 generations by inbreeding. The colony was moved to the Institute of Ophthalmology (University College of London) in 1994, and then the Institute Neurology (University College of London) in 2000 prior to being moved to Queen Mary University of London (2006). The line was re-derived by caesarian section approximately 6 times by Baker. The strain was kept in closed colonies of 5-30 breeding pairs rather than being inbred over a period of nearly 30 years.

A pooled stock colony was established at The Jackson Laboratory in December, 2012. Inbred crosses may be initiated in 2015.

Allele

MGI accession ID: MGI:95894
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Marker synonyms: H-2; MHC-II
Chromosome: 17