These mice carry an ENU-induced point mutation in the Mybl1 gene that results in homozygous male infertility due to meiotic arrest. MYBL1repro9 protein is reduced but not absent in mutant testes.
Dr. Ewelina Bolcun-Filas, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Mybl1 | myeloblastosis oncogene-like 1 |
Mybl1 (myeloblastosis oncogene-like 1; also called A-Myb) is a transcription factor that has been identified as a male-specific master regulator of several crucial meiotic processes.
These Mybl1repro9 mice carry an ENU-generated point mutation (A213E) associated with homozygous male infertility. Testes exhibit a histopathological phenotype indistinguishable from that of Mybl1 knockout animals. Spermatocytes demonstrate subtle defects in autosome synapsis in pachynema, a high incidence of unsynapsed sex chromosomes, incomplete double-strand break repair on synapsed pachytene chromosomes, and a lack of crossing over. Homozygous females are fertile and viability is unaffected.
Homozygous Mybl1repro9 mice do not exhibit the runting or abnormal breast development observed in homozygous knockout mice. This indicates that the mutation is a hypomorphic or separation-of-function allele that specifically impairs the spermatogenesis function of MYBL1. Western blot indicates that MYBL1repro9 protein is reduced, but not absent in mutant testes.
Male C57BL/6J (B6) mice were mutagenized with ethylnitrosourea (ENU) then bred to C3HeB/FeJ (C3H) females to create G1 male founders at The Jackson Laboratory. These males were backcrossed to C3H females, and G2 daughters were backcrossed to the G1 male to yield G3 offspring that were tested for infertility. Genome scans revealed a C to A transversion that results in a glutamic acid to alanine amino acid change at position 213 (A213E). This strain was backcrossed to C57BL/6J for 11 generations by the donating laboratory at Cornell University, then backcrossed for one generation to re-derive stock at The Jackson Laboratory.
Allele Name | reproductive mutant 9, JAX Reproductive Mutagenesis Program |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | G1-419-6 |
Gene Symbol and Name | Mybl1, myeloblastosis oncogene-like 1 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 1 |
Molecular Note | ENU mutagenesis induces a C to A transversion that results in the amino acid substitution of glutamic acid for alanine at position 213 (A213E). |
Homozygous males are infertile due to meiotic arrest. Homozygous females and heterozygous males are fertile. Viability is unaffected.
When using the repro9 mouse strain in a publication, please cite the originating article(s) and include JAX stock #027889 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Mybl1<repro9> |
Frozen Mouse Embryo | B6(C3)-Mybl1<repro9>/JEbfJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(C3)-Mybl1<repro9>/JEbfJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(C3)-Mybl1<repro9>/JEbfJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(C3)-Mybl1<repro9>/JEbfJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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