C.FVB-Tg(Gnrhr-luc/EGFP)L8Mrln/LmwJ

Stock number: 027848
Product name: Glowing Head on BALB/c

Description

These Glowing Head transgenic mice on the BALB/c background are immunologically tolerant to firefly luciferase and Enhanced Green Fluorescent protein, and can be used as a host for labeled syngeneic tumor transplantation.

Detailed description

These “Glowing Head” (GH) transgenic mice express the ffLuc-eGFP (firefly luciferase-Enhanced Green Fluorescent Protein) fusion gene under the control of the rat growth hormone gene promoter. Transgene expression is targeted to the non-immune privileged anterior pituitary and does not interfere with pituitary function, as assessed by serum growth hormone levels. These transgenic mice are immune-tolerant to firefly luciferase and Enhanced Green Fluorescent protein, and can be used as an allograft recipient for labeled syngeneic tumor transplantation, avoiding the effects of xenobiotic/antigenic reporters on testing therapeutic agents. Specifically, this strain may be used with the 4T1 metastatic mammary tumor cell line. Mice that are hemizygous for the transgene and on the BALB/c background are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous on the BALB/c background.

This “Glowing Head” transgenic is also available on a FVB/N genetic background: Stock No. 027665, and a C57BL/6 genetic background: Stock No. 027662.

Development

A transgenic construct containing the ffLuc-eGFP (firefly luciferase-Enhanced Green Fluorescent Protein) fusion gene under the control of the rat growth hormone gene promoter was injected into FVB/N blastocysts. Founder line L8 was subsequently established. The mice were backcrossed to BALB/cANCr for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to BALB/cByJ (Stock No. 001026) at least once to establish the colony.

Allele

Symbol: Tg(Gnrhr-luc/EGFP)L8Mrln
Name: transgene insertion L8, Glenn Merlino
MGI accession ID: MGI:5641258
Generation method: Transgenic
Attributes: Reporter
Synonyms: GH; Glowing Head; rGH-ffLuc-eGFP
Marker symbol: Tg(Gnrhr-luc/EGFP)L8Mrln
Marker name: transgene insertion L8, Glenn Merlino
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: luc,luciferase,firefly; GFP,Green Fluorescent Protein,
Site of expression: The firefly luciferase-eGFP fusion gene is expressed in the anterior pituitary gland and testes.
Molecular note: The transgenic construct contains a firefly luciferase (ffLuc)-eGFP fusion gene under the control of the rat growth hormone gene promoter and human growth hormone sequences. Reporter activity (by bioluminescent imaging) is detected in the anterior pituitary gland and testes.