These Glowing Head transgenic mice on the BALB/c background are immunologically tolerant to firefly luciferase and Enhanced Green Fluorescent protein, and can be used as a host for labeled syngeneic tumor transplantation.
Dr. Glenn T. Merlino, National Institutes of Health
Lalage M. Wakefield, National Cancer Institute
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These “Glowing Head” (GH) transgenic mice express the ffLuc-eGFP (firefly luciferase-Enhanced Green Fluorescent Protein) fusion gene under the control of the rat growth hormone gene promoter. Transgene expression is targeted to the non-immune privileged anterior pituitary and does not interfere with pituitary function, as assessed by serum growth hormone levels. These transgenic mice are immune-tolerant to firefly luciferase and Enhanced Green Fluorescent protein, and can be used as an allograft recipient for labeled syngeneic tumor transplantation, avoiding the effects of xenobiotic/antigenic reporters on testing therapeutic agents. Specifically, this strain may be used with the 4T1 metastatic mammary tumor cell line. Mice that are hemizygous for the transgene and on the BALB/c background are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous on the BALB/c background.
This “Glowing Head” transgenic is also available on a FVB/N genetic background: Stock No. 027665, and a C57BL/6 genetic background: Stock No. 027662.
A transgenic construct containing the ffLuc-eGFP (firefly luciferase-Enhanced Green Fluorescent Protein) fusion gene under the control of the rat growth hormone gene promoter was injected into FVB/N blastocysts. Founder line L8 was subsequently established. The mice were backcrossed to BALB/cANCr for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to BALB/cByJ (Stock No. 001026) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | luc, luciferase, firefly |
Site of Expression | The firefly luciferase-eGFP fusion gene is expressed in the anterior pituitary gland and testes. |
Allele Name | transgene insertion L8, Glenn Merlino |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | GH; Glowing Head; rGH-ffLuc-eGFP |
Gene Symbol and Name | Tg(Gnrhr-luc/EGFP)L8Mrln, transgene insertion L8, Glenn Merlino |
Gene Synonym(s) | |
Promoter | Gnrhr, gonadotropin releasing hormone receptor, rat |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | luc, luciferase, firefly |
Site of Expression | The firefly luciferase-eGFP fusion gene is expressed in the anterior pituitary gland and testes. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgenic construct contains a firefly luciferase (ffLuc)-eGFP fusion gene under the control of the rat growth hormone gene promoter and human growth hormone sequences. Reporter activity (by bioluminescent imaging) is detected in the anterior pituitary gland and testes. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to BALB/cByJ inbred mice (Stock No. 001026). The Donating Investigator has not attempted to make the strain homozygous on the BALB/c background.
When using the Glowing Head on BALB/c mouse strain in a publication, please cite the originating article(s) and include JAX stock #027848 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Gnrhr-luc/EGFP)L8Mrln |
Frozen Mouse Embryo | C.FVB-Tg(Gnrhr-luc/EGFP)L8Mrln/LmwJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C.FVB-Tg(Gnrhr-luc/EGFP)L8Mrln/LmwJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C.FVB-Tg(Gnrhr-luc/EGFP)L8Mrln/LmwJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C.FVB-Tg(Gnrhr-luc/EGFP)L8Mrln/LmwJ Frozen Embryo | $3373.50 |
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