| Allele Name | transgene insertion 1, Jorge Ferrer |
| Allele Type | Transgenic (Recombinase-expressing, Inducible) |
| Allele Synonym(s) | Hnf1bCreER |
| Gene Symbol and Name | Tg(Hnf1b-cre/ERT2)1Jfer, transgene insertion 1, Jorge Ferrer |
| Gene Synonym(s) | |
| Promoter | Hnf1b, HNF1 homeobox B, mouse, laboratory |
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| Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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| Site of Expression | Cre-ERT2 fusion protein is expressed in Hnf1b -expressing cells in pancreatic duct epithelium, hepatic ductal epithelium, kidney, and gut. |
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| Strain of Origin | C57BL/6J x SJL |
| Chromosome | UN |
| Molecular Note | A BAC clone containing the Hnf1b locus (RP23-304H7) was modified by replacing exon 1 with a targeting vector containing an Hnf1b promoter fragment, cre/ERT2, IRES, d2EGFP (a destabilized variant of EGFP), the b-globin polyadenylation site, a FRT/neo/FRT cassette, and an intron 1 fragment that flanks exon 1 of Hnf1b. The targeting vector was electroporated into EL250 cells and homologous recombination was induced. Correctly targeted clones were treated to activate inducible Flp recombinase, removing the FRT/neo/FRT cassette. The recombined BAC was digested, linearized and microinjected into pronuclei of C57BL6/SJL eggs. 10 lines were generated, and after crossing to Rosa26 reporter mice, one line carrying a single copy of the transgene was maintained (after characterization of 4 lines exhibiting similar reporter recombination patterns.) |
