C57BL/6J-Maf1^em1Lnh/J

Stock number: 027668
Product name: Maf1 ZFN (+T)
Research areas: Diabetes and Obesity Research, Metabolism Research, Research Tools

Description

This Maf1 ZFN (zinc finger nuclease) targeted mutation strain exhibits resistance to diet induced obesity. These mice may be suitable for use in studies related to metabolic homeostasis and obesity.

Detailed description

MAF1 homolog (S. cerevisiae) responds to cellular nutrient limitation and cell stress by repressing RNA polymerase III transcription, thus regulating metabolic efficiency. These mice carry a zinc finger nuclease (ZFN) mediated single base pair insertion in the Maf1 gene causing early translational termination (shortly after Thr64). Homozygotes exhibit a phenotype similar to the phenotype observed in mice homozygous for a null allele. Mutant mice are resistant to diet induced obesity. Mice that are homozygous for the targeted mutation are viable and fertile, but may produce fewer pups and have fewer birth events.

Development

Zinc finger nuclease (ZFN) editing induced a single base pair mutation in the Maf1 gene. The resulting change in reading frame causes a termination of translation after Thr64. ZFN mRNA was injected into C57BL/6J oocytes. Founder mice were maintained on the C57BL/6J background. Upon arrival, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Maf1^em1Lnh
Name: endonuclease-mediated mutation 1, Nouria Hernandez
MGI accession ID: MGI:5661490
Generation method: Endonuclease-mediated
Attributes: Null/Knockout
Marker symbol: Maf1
Marker name: MAF1 homolog, negative regulator of RNA polymerase III
Chromosome: 15
Strain of origin: C57BL/6J
Molecular note: Zinc finger nuclease (ZFN) editing induced a single base pair mutation in the Maf1 gene. The resulting change in reading frame causes a termination of translation after Thr64.