This Maf1 ZFN (zinc finger nuclease) targeted mutation strain exhibits resistance to diet induced obesity. These mice may be suitable for use in studies related to metabolic homeostasis and obesity.
Nouria Hernandez, CIG - University of Lausanne
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Endonuclease-mediated (Null/Knockout) | Maf1 | MAF1 homolog, negative regulator of RNA polymerase III |
MAF1 homolog (S. cerevisiae) responds to cellular nutrient limitation and cell stress by repressing RNA polymerase III transcription, thus regulating metabolic efficiency.
These mice carry a zinc finger nuclease (ZFN) mediated single base pair insertion in the Maf1 gene causing early translational termination (shortly after Thr64). Homozygotes exhibit a phenotype similar to the phenotype observed in mice homozygous for a null allele. Mutant mice are resistant to diet induced obesity. Mice that are homozygous for the targeted mutation are viable and fertile, but may produce fewer pups and have fewer birth events.
Zinc finger nuclease (ZFN) editing induced a single base pair mutation in the Maf1 gene. The resulting change in reading frame causes a termination of translation after Thr64. ZFN mRNA was injected into C57BL/6J oocytes. Founder mice were maintained on the C57BL/6J background. Upon arrival, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | endonuclease-mediated mutation 1, Nouria Hernandez |
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Allele Type | Endonuclease-mediated (Null/Knockout) |
Allele Synonym(s) | Maf1 +T |
Gene Symbol and Name | Maf1, MAF1 homolog, negative regulator of RNA polymerase III |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 15 |
Molecular Note | Zinc finger nuclease (ZFN) editing induced a single base pair mutation in the Maf1 gene. The resulting change in reading frame causes a termination of translation after Thr64. |
When maintaining a live colony, these mice can be bred as homozygotes. However, homozygotes may exhibit reduced
fertility and fecundity (may produce fewer pups and have fewer birth events).
When using the Maf1 ZFN (+T) mouse strain in a publication, please cite the originating article(s) and include JAX stock #027668 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Maf1<em1Lnh> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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