Grp-/- KO mice have a neo cassette replacing exon 1 of the gastrin releasing peptide (Grp) gene, abolishing gene expression. GRP is a neurotransmitter released by sensory neurons to initiate itch-related signals in acute and chronic itch. Mice homozygous for this allele are viable and fertile. In a model of spontaneous scratching behavior in BRAFNav1.8 mice, BRAFNav1.8 mice lacking GRP expression do not engage in spontaneous scratching behavior.
A targeting vector was designed to replace exon 1 of the gastrin releasing peptide (Grp) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129X1/SvJ-derived GSI-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females. These Grp-/- mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, James F Battey|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Grp, gastrin releasing peptide|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Exon 1 was replaced with a neo cassette. Immunostaining analysis confirmed the absence of expression in dorsal root ganglion and spinal cord neurons.|
When maintaining a live colony, homozygous mice may be bred together.
When using the GRP KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #027593 in your Materials and Methods section.