IL-23R Tg mice overexpress IL-23R in T cells. In an allergen-induced asthma model, mice exhibit increased airway infiltration of eosinophils and T helper 2 cytokine production. These transgenics mice may be useful in studies related to inflammatory diseases.
Chen Dong, MD Anderson Cancer Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
IL-23R Tg mice overexpress interleukin 23 receptor (Il23r) cDNA, amplified from a T helper (Th) 17 cell pool, in T cells under control of the human CD2 promoter. IL-23R Homozygotes are viable and fertile. IL-23R is the receptor for the cytokine, IL-23, which is primarily secreted by activated dendritic cells, monocytes, and macrophages, and functions in innate and adaptive immunity. It has been shown to be important in some inflammatory diseases including experimental autoimmune encephalitis, collagen-induced arthritis, and intestinal inflammation, as well as tumorigenesis. Mutations in IL-23R are an important susceptibility factor for these disorders. IL-23R Tg mice exhibit increased airway infiltration of eosinophils and T helper (Th) 2 cytokine production, including IL-4, IL-5, and IL-13. IL-23 signaling regulates allergic asthma through modulation of Th2 cell differentiation.
A transgenic vector was generated with interleukin 23 receptor (Il23r) cDNA, amplified from a T helper (Th) 17 cell pool, followed by polyadenylation signal (polyA), under control of a human CD2 promoter. This construct also contains CD2 locus control region (LCR) to increase gene expression by preventing the down-modulation of the expressed gene. The construct was microinjected into C57BL/6J fertilized oocytes, and founder mice were bred to C57BL/6J. IL-23R Tg mice from founder line 2 were identified to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Expressed Gene | Il23r, interleukin 23 receptor, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 2, Chen Dong |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | IL-23R Tg |
Gene Symbol and Name | Tg(CD2-Il23r)2Cdon, transgene insertion 2, Chen Dong |
Gene Synonym(s) | |
Promoter | CD2, CD2 molecule, human |
Expressed Gene | Il23r, interleukin 23 receptor, mouse, laboratory |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | A transgenic vector was generated with interleukin 23 receptor (Il23r) cDNA, amplified from a T helper (Th) 17 cell pool, followed by polyadenylation signal (polyA), under control of a human CD2 promoter. This construct also contains the CD2 locus control region (LCR) to increase gene expression by preventing the down-modulation of the expressed gene. Line 2 was analyzed. |
When maintaining a live colony, transgenic mice may be bred together.
When using the IL-23R Tg mouse strain in a publication, please cite the originating article(s) and include JAX stock #027591 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(CD2-Il23r)2Cdon |
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