B6.Cg-Tg(HIV-luc)27Tsb/J

Stock number: 027547
Product name: HLL (HIV-LTR/Luciferase)
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These HLL (HIV-LTR/Luciferase) mice act as a luciferase reporter for NF-κB activation and inflammatory responses.

Detailed description

These HLL transgenic mice serve as a reporter of NF-κB activity and inflammation, by virtue of their expressing NF-κB-driven luciferase. The transgenic construct contains firefly luciferase under the control of the proximal 5’ human immunodeficiency virus (HIV-1) long terminal repeat (LTR), which requires NF-κB activity for transcription. LPS challenge confirmed luciferase expression reflects NF-κB activation in a temporal, spatial and organ -specific manner. Mice that are hemizygous for the transgene are viable and fertile. The Donating Investigator has not attempted to make this strain homozygous. Differences in expression levels between hemizygotes and homozygotes have not yet been determined (July 2015). During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.

Development

A transgenic construct (designed by Dr. Lawrence Kerr in 1995) containing 0.68kb sequence from the proximal HIV-long terminal repeat upstream of the luciferase coding region (in the pGL2-basic plasmid vector) was injected into fertilized mouse eggs. Founder line 27 was subsequently established on a C57BL/6 and DBA background. The Donating Investigator intercrossed hemizygotes to produce homozygotes. The mice were then backcrossed to C57BL/6 for 10 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(HIV-luc)27Tsb
Name: transgene insertion 27, Timothy S Blackwell
MGI accession ID: MGI:5660354
Generation method: Transgenic
Attributes: Reporter
Synonyms: HLL
Marker symbol: Tg(HIV-luc)27Tsb
Marker name: transgene insertion 27, Timothy S Blackwell
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: luc,luciferase,firefly
Molecular note: The transgenic construct contains a cDNA encoding firefly luciferase under the control of tandem copies of a 36-base enhancer from the 5' HIV-long terminal repeat (containing two NF-kappaB binding sites, GGGACTTTCC) upstream of a HSV (herpes simplex virus) minimal thymidine kinase promoter.