Retro mice contain an ENU-induced A to G transition of nucleotide 1304 in exon 2 of the Lexm (lymphocyte expansion molecule) gene. Homozygous mice are viable and fertile. Retro mice exhibit increased antigen-dependent CD8+ T cell proliferation, effector function, and memory cell generation in response to infection with lymphocytic choriomeningitis virus. They have a 10-fold increase in CD8 T cells specific for LCMV np396 peptide with increased CTL activity in ex vivo killing experiments including increased exocytosis of cytotoxic granules and increased production of interferon (IFN)-γ. Retro mice succumb to LCMV C13 infection after 14 days presumably due to an elevation in CTL-mediated cytolysis resulting in the fatal loss of vascular integrity.
Following multidose N-ethyl-N-nitrosourea (ENU) treatments to induce mutations in founder C57BL/6J mice, a forward genetic screen was utilized to identify a family of Retro mice, containing increased levels of LCMV-specific CD8 T cells after LCMV C13 infection. Using high-throughput exome sequencing, a mutation was identified in the Lexm (Lymphocyte Expansion Molecule) gene. Sequencing identified an A to G transition of nucleotide 1304 in exon 2 of Lem. These Retro mice were bred to littermates or to C57BL/6J mice to maintain a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation.
|Allele Type||Chemically induced (ENU) (Not Applicable)|
|Gene Symbol and Name||Lexm, lymphocyte expansion molecule|
|Strain of Origin||C57BL/6J|
|Molecular Note||This ENU-induced mutation was isolated in a screen for cytotoxic T lymphocyte response to LCMV C13 infection. Sequencing identified an A to G transition at nucleotide 1304 in exon 2. Western blot analysis reveals increased protein expression on total CD8+ T cells following LCMV infection.|
When maintaining a live colony homozygous mice may be bred together.
When using the Retro mouse strain in a publication, please cite the originating article(s) and include JAX stock #027544 in your Materials and Methods section.