C57BL/6J-Cimap2^Retro/J

Stock number: 027544
Product name: Retro
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

ENU-induced Retro mice may be useful in studying CD8⁺ T cell–mediated immunity.

Detailed description

Retro mice contain an ENU-induced A to G transition of nucleotide 1304 in exon 2 of the Cimap2 (ciliary microtubule associated protein 2, formerly Lexm) gene. Homozygous mice are viable and fertile. Retro mice exhibit increased antigen-dependent CD8+ T cell proliferation, effector function, and memory cell generation in response to infection with lymphocytic choriomeningitis virus. They have a 10-fold increase in CD8 T cells specific for LCMV np396 peptide with increased CTL activity in ex vivo killing experiments including increased exocytosis of cytotoxic granules and increased production of interferon (IFN)-γ. Retro mice succumb to LCMV C13 infection after 14 days presumably due to an elevation in CTL-mediated cytolysis resulting in the fatal loss of vascular integrity.

Development

Following multidose N-ethyl-N-nitrosourea (ENU) treatments to induce mutations in founder C57BL/6J mice, a forward genetic screen was utilized to identify a family of Retro mice, containing increased levels of LCMV-specific CD8 T cells after LCMV C13 infection. Using high-throughput exome sequencing, a mutation was identified in the Cimap2 (ciliary microtubule associated protein 2, formerly Lexm) gene. Sequencing identified an A to G transition of nucleotide 1304 in exon 2 of Cimap2. These Retro mice were bred to littermates or to C57BL/6J mice to maintain a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation.

Allele

Symbol: Cimap2^Retro
Name: Retro
MGI accession ID: MGI:5694208
Generation method: Chemically induced (ENU)
Attributes: Not Applicable
Marker symbol: Cimap2
Marker name: ciliary microtubule associated protein 2
Chromosome: 4
Strain of origin: C57BL/6J
Molecular note: This ENU-induced mutation was isolated in a screen for cytotoxic T lymphocyte response to LCMV C13 infection. Sequencing identified an A to G transition at nucleotide 1304 in exon 2. Western blot analysis reveals increased protein expression on total CD8+ T cells following LCMV infection.