These NGL (NF-κB-GFP-luciferase) mice act as an EGFP-luciferase reporter for cellular localization of NF-κB activation and quantification.
Timothy S Blackwell, Vanderbilt University Medical Center
Genetic Background | Generation |
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N10+pN1F8
|
Allele Type |
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Transgenic (Reporter) |
These NGL (NF-κB-GFP-luciferase) transgenic mice act as a NF-κB activity reporter, expressing an NF-κB driven enhanced green fluorescent protein-luciferase fusion protein.
The EGFP allows cellular localization of NF-κB positive cells and the luciferase allows non-invasive in vivo bioluminescence imaging of NF-κB activity in cells.
LPS challenge confirmed GFP-luciferase expression reflects NF-κB activation. Mice that are homozygous for the NF-kB reporter transgene are viable and fertile. Differences in expression levels between hemizygotes and homozygotes have not yet been determined (July 2015).
During backcrossing, the Y chromosome may not have been fixed to the FVB genetic background.
A transgenic construct containing an enhanced
GFP-luciferase fusion gene under the control of tandem copies of a 36-base enhancer from the 5' HIV-long terminal repeat (containing two NF-κB binding sites, GGGACTTTCC) upstream of a HSV (herpes simplex virus) minimal thymidine kinase promoter, was injected into fertilized mouse eggs. Founder line 8 was subsequently established on a C57BL/6 and DBA background. The mice were crossed to C57BL/6 and then backcrossed to FVB for approximately 10 generations. During backcrossing, the Y chromosome may not have been fixed to the FVB genetic background. Upon arrival at The Jackson Laboratory, these NFkB reporter mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | luc, luciferase, firefly |
Site of Expression | The EGFP-luciferase fusion protein is expressed in a variety of lung cells. |
Allele Name | transgene insertion 8, Timothy S Blackwell |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | NF-kappaB-GFP-Luciferase (NGL); NLG |
Gene Symbol and Name | Tg(HIV-EGFP,luc)8Tsb, transgene insertion 8, Timothy S Blackwell |
Gene Synonym(s) | |
Promoter | HIV, Human immunodeficiency virus 1, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | luc, luciferase, firefly |
Site of Expression | The EGFP-luciferase fusion protein is expressed in a variety of lung cells. |
Strain of Origin | C57BL/6 or DBA/2 |
Chromosome | UN |
Molecular Note | Four tandem copies of a 36-base enhancer from the 5' HIV-long terminal repeat (containing two NF-kappaB binding sites, GGGACTTTCC) were placed upstream of the HSV minimal thymidine kinase promoter. This enhancer-promoter construct was used to drive expression of an enhanced GFP-luciferase fusion protein. |
When maintaining a live colony, these NFkB reporter mice can be bred as homozygotes.
When using the NGL (NF-κB-GFP-luciferase) mouse strain in a publication, please cite the originating article(s) and include JAX stock #027529 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(HIV-EGFP,luc)8Tsb |
Frozen Mouse Embryo | FVB.Cg-Tg(HIV-EGFPluc)8Tsb/J | $2595.00 |
Frozen Mouse Embryo | FVB.Cg-Tg(HIV-EGFPluc)8Tsb/J | $2595.00 |
Frozen Mouse Embryo | FVB.Cg-Tg(HIV-EGFPluc)8Tsb/J | $3373.50 |
Frozen Mouse Embryo | FVB.Cg-Tg(HIV-EGFPluc)8Tsb/J | $3373.50 |
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