FVB.Cg-Tg(HIV-EGFP,luc)8Tsb/J

Stock number: 027529
Product name: NGL (NF-κB-GFP-luciferase)
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These NGL (NF-κB-GFP-luciferase) mice act as an EGFP-luciferase reporter for cellular localization of NF-κB activation and quantification.

Detailed description

These NGL (NF-κB-GFP-luciferase) transgenic mice act as a NF-κB activity reporter, expressing an NF-κB driven enhanced green fluorescent protein-luciferase fusion protein. The EGFP allows cellular localization of NF-κB positive cells and the luciferase allows non-invasive in vivo bioluminescence imaging of NF-κB activity in cells. LPS challenge confirmed GFP-luciferase expression reflects NF-κB activation. Mice that are homozygous for the NF-kB reporter transgene are viable and fertile. Differences in expression levels between hemizygotes and homozygotes have not yet been determined (July 2015). During backcrossing, the Y chromosome may not have been fixed to the FVB genetic background.

Development

A transgenic construct containing an enhanced GFP-luciferase fusion gene under the control of tandem copies of a 36-base enhancer from the 5' HIV-long terminal repeat (containing two NF-κB binding sites, GGGACTTTCC) upstream of a HSV (herpes simplex virus) minimal thymidine kinase promoter, was injected into fertilized mouse eggs. Founder line 8 was subsequently established on a C57BL/6 and DBA background. The mice were crossed to C57BL/6 and then backcrossed to FVB for approximately 10 generations. During backcrossing, the Y chromosome may not have been fixed to the FVB genetic background. Upon arrival at The Jackson Laboratory, these NFkB reporter mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Allele

Symbol: Tg(HIV-EGFP,luc)8Tsb
Name: transgene insertion 8, Timothy S Blackwell
MGI accession ID: MGI:5568390
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(HIV-EGFP,luc)8Tsb
Marker name: transgene insertion 8, Timothy S Blackwell
Chromosome: UN
Strain of origin: C57BL/6 or DBA/2
Expressed genes: GFP,Green Fluorescent Protein,; luc,luciferase,firefly
Site of expression: The EGFP-luciferase fusion protein is expressed in a variety of lung cells.
Molecular note: Four tandem copies of a 36-base enhancer from the 5' HIV-long terminal repeat (containing two NF-kappaB binding sites, GGGACTTTCC) were placed upstream of the HSV minimal thymidine kinase promoter. This enhancer-promoter construct was used to drive expression of an enhanced GFP-luciferase fusion protein.