FVB.Cg-Tg(HIV-EGFP,luc)8Tsb/J

Stock number: 027529
Product name: NGL (NF-κB-GFP-luciferase)
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.

These NGL (NF-κB-GFP-luciferase) mice act as an EGFP-luciferase reporter for cellular localization of NF-κB activation and quantification.

Detailed description

These NGL (NF-κB-GFP-luciferase) transgenic mice act as a NF-κB activity reporter, expressing an NF-κB driven enhanced green fluorescent protein-luciferase fusion protein. The EGFP allows cellular localization of NF-κB positive cells and the luciferase allows non-invasive in vivo bioluminescence imaging of NF-κB activity in cells. LPS challenge confirmed GFP-luciferase expression reflects NF-κB activation. Mice that are homozygous for the NF-kB reporter transgene are viable and fertile. Differences in expression levels between hemizygotes and homozygotes have not yet been determined (July 2015). During backcrossing, the Y chromosome may not have been fixed to the FVB genetic background.

Development

A transgenic construct containing an enhanced GFP-luciferase fusion gene under the control of tandem copies of a 36-base enhancer from the 5' HIV-long terminal repeat (containing two NF-κB binding sites, GGGACTTTCC) upstream of a HSV (herpes simplex virus) minimal thymidine kinase promoter, was injected into fertilized mouse eggs. Founder line 8 was subsequently established on a C57BL/6 and DBA background. The mice were crossed to C57BL/6 and then backcrossed to FVB for approximately 10 generations. During backcrossing, the Y chromosome may not have been fixed to the FVB genetic background. Upon arrival at The Jackson Laboratory, these NFkB reporter mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Allele

Symbol: Tg(HIV-EGFP,luc)8Tsb
Name: transgene insertion 8, Timothy S Blackwell
MGI accession ID: MGI:5568390
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(HIV-EGFP,luc)8Tsb
Marker name: transgene insertion 8, Timothy S Blackwell
Chromosome: UN
Strain of origin: C57BL/6 or DBA/2
Expressed genes: GFP,Green Fluorescent Protein,; luc,luciferase,firefly
Site of expression: The EGFP-luciferase fusion protein is expressed in a variety of lung cells.
Molecular note: Four tandem copies of a 36-base enhancer from the 5' HIV-long terminal repeat (containing two NF-kappaB binding sites, GGGACTTTCC) were placed upstream of the HSV minimal thymidine kinase promoter. This enhancer-promoter construct was used to drive expression of an enhanced GFP-luciferase fusion protein.