These AND-34 knock-out mice exhibit lens abnormalities. They are suitable for use in applications related to the study of focal adhesion adaptor proteins, cell adhesion to extracellular matrix proteins and Src signaling.
Adam Lerner, Boston University School of Medicine
The Bcar3 gene encodes an adapter protein that binds to focal adhesion adaptor proteins such as p130CAS/BCAR1. In human breast cancer cells, BCAR3 is part of the intracellular anti-estrogen resistance signal transduction pathway. These mice carry a knock out allele of the Bcar3 gene in which exon 4 and most of exon 5 have been replaced by a NEO selection cassette resulting in loss of the SH2 region. Mice that are homozygous for the targeted mutation are viable and fertile. No gene product (protein) is detected by Western blot analysis of splenic lymphocytes. Homozygotes exhibit opaque, irregular eye lenses.
During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
A targeting vector containing a NEO cassette was used to disrupt exon 4 and most of exon 5.
The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J female mice, and then backcrossed to C57BL/6J for 10 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Adam Lerner|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Bcar3, breast cancer anti-estrogen resistance 3|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exon 4 and most of exon 5 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis of splenic lymphocyte extracts.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the BCAR3KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #027525 in your Materials and Methods section.