IL22Cre mice have a codon optimized Cre Recombinase (iCre), replacing exon 1 of the Il22 (interleukin 22) gene. The presence of iCre abolishes expression of IL-22. IL-22 is a cytokine expressed by immune cells that is required for immune responses to bacterial infections. Homozygous mice are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring. When bred to mice containing a cre-inducible fluorophore, mice serve as a fate reporter that marks cells that had initiated the IL-22 program. Under steady state conditions, iCre expression is restricted to group 3 innate lymphoid cells (ILC3) in the gut and &gamma;&delta; T cells in skin or lung. &gamma;&delta; and CD4 T cells expand in the skin when used as a model of psoriasiform inflammation induced by the TLR7 ligand, imiquimod. An expansion of CD4 T cells, which were induced in innate lymphoid follicles in the colon, was evident during Citrobacter rodentium infection. Homozygous IL-22 deficient mice fail to clear the infection and succumb to the infection by day 8.

IL22Cre knock-out/in mice express Cre recombinase in a subset of lymphoid cells including group 3 innate lymphoid cells in the gut and &gamma;&delta; T cells in skin or lung.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

<a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> for more information.