IL22Cre knock-out/in mice express Cre recombinase in a subset of lymphoid cells including group 3 innate lymphoid cells in the gut and γδ T cells in skin or lung.
Brigitta Stockinger, The Francis Crick Institute
Genetic Background | Generation |
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N?+pN2F13
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Il22 | interleukin 22 |
IL22Cre mice have a codon optimized Cre Recombinase (iCre), replacing exon 1 of the Il22 (interleukin 22) gene. The presence of iCre abolishes expression of IL-22. IL-22 is a cytokine expressed by immune cells that is required for immune responses to bacterial infections. Homozygous mice are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring. When bred to mice containing a cre-inducible fluorophore, mice serve as a fate reporter that marks cells that had initiated the IL-22 program. Under steady state conditions, iCre expression is restricted to group 3 innate lymphoid cells (ILC3) in the gut and γδ T cells in skin or lung. γδ and CD4 T cells expand in the skin when used as a model of psoriasiform inflammation induced by the TLR7 ligand, imiquimod. An expansion of CD4 T cells, which were induced in innate lymphoid follicles in the colon, was evident during Citrobacter rodentium infection. Homozygous IL-22 deficient mice fail to clear the infection and succumb to the infection by day 8.
A targeting construct was designed to replace exon 1 of the Il22 (interleukin 22) gene with a 6 aa linker sequence, a codon improved Cre Recombinase gene (iCre) followed by an SV40 polyadenylation sequence, and a frt-flanked neomycin resistance (neo) cassette. This construct was electroporated into C57BL/6N-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to FLPe mice on a C57BL/6-NIMR background to remove the neo cassette. These IL22Cre mice were backcrossed for at least 3 generations to C57BL/6-NIMR mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre expression is restricted to group 3 innate lymphoid cells in the gut and gamma/delta T cells in skin or lung. |
Allele Name | targeted mutation 1.1, Brigitta Stockinger |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | IL22Cre |
Gene Symbol and Name | Il22, interleukin 22 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre expression is restricted to group 3 innate lymphoid cells in the gut and gamma/delta T cells in skin or lung. |
Strain of Origin | C57BL/6N |
Chromosome | 10 |
Molecular Note | A targeting construct was designed to replace exon 1 of the Il22 (interleukin 22) gene with a 6 aa linker sequence, a codon improved Cre Recombinase gene (iCre) followed by an SV40 polyadenylation sequence and a FRT-flanked neomycin resistance (neo) cassette. Flp-mediated recombination removed the FRT-flanked neo cassette. |
When maintaining a live colony homozygous mice may be bred together. The donating investigator reports that homozygotes are vulnerable to opportunistic intestinal infections.
When using the IL22Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #027524 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Il22<tm1.1(icre)Stck> |
Frozen Mouse Embryo | C57BL/6-Il22<tm1.1(icre)Stck>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Il22<tm1.1(icre)Stck>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Il22<tm1.1(icre)Stck>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Il22<tm1.1(icre)Stck>/J | $3373.50 |
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