The Env28 TCR transgenic line 6C2.16 has a CD8+ T cell repertoire that is specific for the Ld-restricted ENV28 epitope (hepatitis B virus envelope/surface antigen residues 28-39 [IPQSLDSWWTSL]). These mice may be useful in studying cellular mechanisms of CD8+ T cell immunity, memory and protection from HBV; especially early events in the host-virus relationship that determine the outcome of hepatotropic human-virus infections.
Dr. Francis (Frank) V Chisari, The Scripps Research Institute
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
In humans, the hepatitis B virus (HBV) causes acute and chronic hepatitis and hepatocellular carcinoma. Clearance of HBV requires functional virus-specific CD8+ T cell responses.
The Env28 TCR transgenic line 6C2.16 is a BALB/c-congenic (H-2d) mouse strain carrying T cell receptor α and β transgenes that recognize an Ld-restricted ENV28 epitope (residues 28-39 [IPQSLDSWWTSL] of the HBV envelope/surface antigen [HBsAg]).
These mice have a CD8+ T cell repertoire (greater than 83% of the splenic CD8+ T cells) that is specific for the ENV28 epitope. The ENV28-specific CD8+ T cells are immunologically naive with respect to their cognate antigen.
Hemizygous mice are viable and fertile. To date (July 2015), it has not been attempted to make this strain homozygous.
The Env28 TCR transgenic line 6C2.16 was designed by Dr. Francis V. Chisari (The Scripps Research Institute). To generate the T cell clones, the H-2d mouse P815 mastocytoma target cell line was incubated with the major envelope polypeptide amino acids 21 to 40 from hepatitis B virus (HBV) envelope/surface antigen (HBsAg). The resulting Ld-restricted CD3+ CD4- CD8+ cytotoxic T lymphocytes (CTL) clone "6C2" was found to recognize an epitope located between residues 28-39 of HBsAg (ENV28; IPQSLDSWWTSL). The identity of the T cell receptor (TCR) Vα and Vβ chains on the surface of the "6C2" T cells was found to be Vα4.1 JαNEW and Vβ1.1 Jβ2.5. These TCR sequences were cloned into TCR cassette expression vectors (pTα and pTβ), and the resulting constructs (~15.0 kbp Tα ENV28 6C2 transgene and ~17.4 kbp Tβ ENV28 6C2 transgene) were co-microinjected into fertilized (BALB/cByJ x C57BL/6J)F2 oocytes. Founder mice were bred with BALB/cJ for germline transmission, and mice from line 16 (lineage 6C2.16) were selected. The Env28 TCR transgenic line 6C2.16 colony was backcrossed more than 12 generations with BALB/cJ inbred mice (H-2d). In 2015, albino males hemizygous for the transgene were sent to The Jackson Laboratory Repository. Upon arrival, males were used to cryopreserve sperm. To establish the living mouse colony, an aliquot of the frozen sperm is used to fertilize oocytes from BALB/cJ inbred females (Stock No. 000651).
Of note, the donating investigator reports that, at least once during backcrossing, a hemizygous female was bred to a BALB/cJ inbred male (thus the Y chromosome of the congenic strain is of BALB/cJ origin).
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 16, Francis Chisari |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Env28 TCR transgenic |
Gene Symbol and Name | Tg(Tcra6C2,Tcrb6C2)16Chi, transgene insertion 16, Francis Chisari |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | BALB/cByJ or C57BL/6J |
Chromosome | UN |
General Note | TCR expression cassettes into which the TCRalpha and TCRbeta cDNA sequences had been cloned were co-injected into (BALB/cByJ x C57BL/6J)F2 fertilized oocytes. |
Molecular Note | Rearranged Valpha4.1 JalphaNEW and Vbeta1.1 Jbeta2.5 sequences are obtained from L |
When maintaining our live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to BALB/cJ inbred mice (Stock No. 000651). To date (July 2015), it has not been attempted to make this strain homozygous.
When using the Env28 TCR transgenic line 6C2.16 mouse strain in a publication, please cite the originating article(s) and include JAX stock #027510 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Tcra6C2,Tcrb6C2)16Chi |
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