Slc23a2- KO mice may be useful for studying the transport of ascorbic acid into tissues and across the placenta.
Robert L Nussbaum, University of California San Francisco
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Slc23a2 | solute carrier family 23 (nucleobase transporters), member 2 |
Slc23a2- mice have a neo cassette replacing exons 10-12 of the solute carrier family 23 (nucleobase transporters), member 2 (Slc23a2) gene, abolishing gene function. Slc23a2 encodes an ascorbic acid transporter. Ascorbic acid, or Vitamin C, is required for the stability of collagen and may play a role as an antioxidant against oxidative stress. Heterozygous Slc23a2+/- mice are viable and fertile, while, homozygous mice die soon after birth due to respiratory failure and intracerebral hemorrhage. Reduced levels of ascorbic-acid are seen in most tissues of Slc23a2+/- newborns as compared with wild-type pups. Adult Slc23a2+/- mice have reduced levels in the brain and blood, but not liver. Ascorbic-acid levels are undetectable in the brain, pituitary, adrenals and pancreas of Slc23a2-/- mice, and are reduced in liver, kidney, muscle, and blood. These mice die before initial alveolar expansion, and exhibit intracranial hemorrhaging over the convex surfaces of the brain.
A targeting vector was designed to replace exons 10-12 of the solute carrier family 23 (nucleobase transporters), member 2 (Slc23a2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to 129S6/SvEvTac mice. Resulting Slc23a2- mice were maintained on a 129S6/SvEvTac background. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Robert L Nussbaum |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Slc23a1-/-; SVCT2- |
Gene Symbol and Name | Slc23a2, solute carrier family 23 (nucleobase transporters), member 2 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | 532 nucleotides of the gene spanning exons 10-12 were replaced with a neomycin resistance cassette via homologous recombination resulting in a deletion and frameshift. RT-PCR verified the presence of mutant transcript in brain of homozygous mutant animals. Disruption of protein function was verified via ascorbic-acid uptake assay. Mouse embryonic fibroblasts from homozygous mutant animals exhibit less than 5% of normal ascorbic-acid uptake compared to wild-type. |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Homozygous mice are born but die soon after birth due to respiratory failure and intracerebral hemorrhage.
When using the Slc23a2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #027439 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Slc23a2<tm1Nbm> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.