&#945 2i TG mice express the D157A mutant form of the rat Prkaa2 (protein kinase, AMP-activated, alpha 2 catalytic subunit) cDNA under the direction of the muscle creatine kinase promoter. PRKAA2 is the catalytic subunit of the AMP-activated protein kinase an energy-sensing enzyme that responds to metabolic stress. The D157A amino acid substitution renders the catalytic subunit inactive resulting in almost undetectable AMPK&#945 2 basal activity, and reduced AMPK&#945 1 activity. Hemizygous and homozygous mice are viable and fertile. Known AMPK stimuli AICAR and rotenone, but not muscle contraction or sorbitol, inhibit in vitro glucose transport in extensor digitorum longus muscles. In vivo, contraction-stimulated glucose transport is normal. This strain may be useful for studying mechanisms of glucose transport regulation.

&#945 2i TG mice express the inactive D157A mutant form of the rat Prkaa2 under the direction of the muscle creatine kinase promoter. Loss of AMPK &#945 2 activity results in inhibition of glucose transport in AICAR- and rotenon-stimulated muscle. This strain may be useful for studying mechanisms of glucose transport regulation.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.