Overview

Also Known As:capns1^flox, Calp line

Exons 4 to 9 of the mouse Capns1 (calpain, small subunit 1) gene are flanked by loxP sites in this conditional mutant strain that is useful for studying protein function in neuronal injury.

Donating Investigator

Stan Krajewski, Sanford/Burnham Medical Research Institute

Genetic overview

Genetic Background	Generation

Capns1^tm1Kraj

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed))	Capns1	calpain, small subunit 1

View Genetics

Research Applications

Developmental Biology Research

View All Research Applications

Details

Detailed Description

Calp mutant mice possess loxP sites flanking exons 4-9 of the Capns1 or calpain, small subunit 1 gene. Calpains are calcium-dependent cysteine proteinases involved in multiple cellular functions; in particular they have been implicated in neurodegenerative diseases. Heterozygous mice are viable and fertile, however, homozygous embryos exhibit lethality prior to E10.5. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 4-9 deleted in the cre-expressing tissues. This allele causes in embryonic lethality in the homozygous state.

For example, when bred to mice carrying a pan-neuronal CRE3 transgene, mice exhibit impaired performance in the Morris Water Maze (MWM). This strain may be useful for studying protein function in neuronal injury.

Development

A loxP-flanked neomycin resistance cassette is inserted upstream of exon 4 followed by a loxP site downstream of exon 9. The construct was electroporated into unspecified embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and the resulting chimeric animals were crossed to FVB/N mice for at least 13 generations.

Control Suggestions

001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Genetics

Capns1^tm1Kraj

Allele Symbol: Capns1^tm1Kraj

Allele Name	targeted mutation 1, Stan Krajewski
Allele Type	Targeted (Conditional ready (e.g. floxed))
Allele Synonym(s)	Calp; capns1^flox
Gene Symbol and Name	Capns1, calpain, small subunit 1
Gene Synonym(s)
Strain of Origin	Not Specified
Chromosome	7
Molecular Note	A loxP-flanked neomycin resistance cassette is inserted upstream of exon 4 followed by a loxP site downstream of exon 9.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Embryonic Lethality (Homozygous)

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Capns1^tm1Kraj/Capns1^tm1Kraj
involves: FVB/N

mortality/aging

embryonic lethality during organogenesis
- midgestational embryonic lethality; embryos are dead by E10.5

References

Additional - Capns1^tm1Kraj related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Capns1
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as heterozygotes, specifically the donating investigator recommends heterozygous female x wild-type male. This allele results in embryonic lethality in the homozygous state.
- Additional Breeding and Husbandry Support
Citation
When using the capns1^flox mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37582 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:capns1flox, Calp line

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Capns1tm1Kraj

Allele Symbol: Capns1tm1Kraj

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Capns1tm1Kraj/Capns1tm1Krajinvolves: FVB/N

mortality/aging

References

Additional - Capns1tm1Kraj related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:capns1^flox, Calp line

Capns1^tm1Kraj

Allele Symbol: Capns1^tm1Kraj

Genotype: Capns1^tm1Kraj/Capns1^tm1Kraj
involves: FVB/N

Additional - Capns1^tm1Kraj related