Exons 4 to 9 of the mouse Capns1 (calpain, small subunit 1) gene are flanked by loxP sites in this conditional mutant strain that is useful for studying protein function in neuronal injury.
Stan Krajewski, Sanford/Burnham Medical Research Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed)) | Capns1 | calpain, small subunit 1 |
Calp mutant mice possess loxP sites flanking exons 4-9 of the Capns1 or calpain, small subunit 1 gene. Calpains are calcium-dependent cysteine proteinases involved in multiple cellular functions; in particular they have been implicated in neurodegenerative diseases. Heterozygous mice are viable and fertile, however, homozygous embryos exhibit lethality prior to E10.5. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 4-9 deleted in the cre-expressing tissues. This allele causes in embryonic lethality in the homozygous state.
For example, when bred to mice carrying a pan-neuronal CRE3 transgene, mice exhibit impaired performance in the Morris Water Maze (MWM). This strain may be useful for studying protein function in neuronal injury.
A loxP-flanked neomycin resistance cassette is inserted upstream of exon 4 followed by a loxP site downstream of exon 9. The construct was electroporated into unspecified embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and the resulting chimeric animals were crossed to FVB/N mice for at least 13 generations.
Allele Name | targeted mutation 1, Stan Krajewski |
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Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | Calp; capns1flox |
Gene Symbol and Name | Capns1, calpain, small subunit 1 |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 7 |
Molecular Note | A loxP-flanked neomycin resistance cassette is inserted upstream of exon 4 followed by a loxP site downstream of exon 9. |
While maintaining a live colony, these mice are bred as heterozygotes, specifically the donating investigator recommends heterozygous female x wild-type male. This allele results in embryonic lethality in the homozygous state.
When using the capns1flox mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37582 in your Materials and Methods section.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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