Pex10CY mice carry an ENU-generated point mutation of the Pex10 gene, exhibit abnormal neurodevelopment, and may be useful in studies of peroxisomal biogenesis disorders.
Lee Niswander, University of Colorado, Denver
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (ENU) | Pex10 | peroxisomal biogenesis factor 10 |
Mutations in the human PEX10 gene cause Zellweger spectrum of Peroxisome Biogenesis Disorder types 6A and 6B. These chemically-induced (ENU) mutant mice carry a single G to A base pair change in the Pex10 gene, resulting in a C294Y amino acid change that disrupts the second zinc RING finger binding domain. Most (98%) homozygotes die within hours of birth. Homozygous embryos begin to exhibit reduced locomotion at E17.5, and at birth are cyanoic and have no milk in their stomachs. Surviving homozygotes are significantly smaller in size than wildtype controls and display an ataxic gait. Very long chain fatty acids (VLCFA) levels are approximately 10-fold higher, and plasmalogen levels are approximately 21-fold lower in E18.5 homozygous embryos. Defective axon bundling by Schwann cells and axonal defects are observed in the sciatic nerves of homozygous embryos. Defects in neuromuscular synapses are found in soleus muscles, while deficits in axon extension, abnormal Schwann cell location and fewer Schwann cells in diaphragm muscles are also noted.
Fibroblasts from homozygous embryos exhibit diffuse immunolabeling of the peroxisomal marker, catalase, compared to the punctate immunolabeling pattern observed in fibroblasts from wildtype control mice. During backcrossing, the Y chromosome may not have been fixed to the 129S1/SvImJ genetic background.
Following multidose N-ethyl-N-nitrosourea (ENU) treatments to induce mutations in founder C57BL/6J mice, a forward genetic screen was utilized to identify mice exhibiting embryonic locomotor defects. The founder Pex10CY male was crossed to 129S1/SvImJ wildtype female mice. Sequencing of multiple loci revealed a single base pair change G to A, resulting in the C294Y amino acid change, which disrupts the second zinc RING finger binding domain
encoded by Pex10. The mice were then backcrossed to 129S1/SvImJ for 12 generations. During backcrossing, the Y chromosome may not have been fixed to the 129S1/SvImJ genetic background.
Upon arrival at The Jackson Laboratory, the mice were crossed to 129S1/SvImJ
(Stock No. 002448) at least once to establish the colony.
Allele Name | mutation 1, Lee Niswander |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | Pex10CY |
Gene Symbol and Name | Pex10, peroxisomal biogenesis factor 10 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 4 |
Molecular Note | ENU induced a single base pair change from G to A, that results in the amino acid substitution of a cysteine for tyrosine at position 294 (C294Y), which disrupts the second zinc RING finger binding domain. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to 129S1/SvImJ inbred mice
(Stock No. 002448). Most (98%) homozygotes die within hours of birth.
When using the Pex10CY mouse strain in a publication, please cite the originating article(s) and include JAX stock #027330 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Pex10<m1Nisw> |
Frozen Mouse Embryo | 129S1.B6-Pex10<m1Nisw>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129S1.B6-Pex10<m1Nisw>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129S1.B6-Pex10<m1Nisw>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | 129S1.B6-Pex10<m1Nisw>/J Frozen Embryos | $3373.50 |
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