S-SCAM transgenic mice may be useful for studying the morphological features and sex-dependent behavioral defects associated with Schizophrenia.
Sang H Lee, Medical College of Wisconsin
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice hemizygous for the S-SCAM allele are viable and fertile. Expression of myc-tagged Magi2 is regulated by a calcium/calmodulin-dependent protein kinase II alpha (CaMKIIα) promoter, which leads to overexpression of Magi2 (Synaptic Scaffolding Molecule; S-SCAM) in the excitatory neurons of the forebrain from postnatal stages to the adult stage. S-SCAM encodes a postsynaptic scaffolding protein involved in the synaptic protein organization and maintenance of synaptic AMPA receptor levels. Mutations and duplications (rare copy number variations (CNVs)) of S-SCAM have been associated with the pathogenesis of the psychiatric disorder Schizophrenia (SZ).
S-SCAM mice express myc-S-SCAM specifically in the forebrain, no expression is seen in the cerebellum. These Tg mice show a 1.5-fold increase in the expression of S-SCAM protein. They exhibit morphological features and sex-dependent selective behavioral deficits, similar to those seen in individuals with SZ. S-SCAM Tg mice have an increased size of lateral ventricles and a reduced number of parvalbumin-stained neurons. They show enhanced excitatory synaptic transmission and elevated synaptic strength, which is detrimental to the induction of normal synaptic plasticity in males. As such, male S-SCAM Tg mice showed significantly impaired long term potential (LTP) hyperglutamatergic function due to a partial saturation of synaptic AMPA receptors. Mice exhibit hyperlocomotor activity, deficits in prepulse inhibition, increased anxiety, impaired social interaction, and working memory deficit.
A transgenic vector was generated including a 3.9 kb myc-tagged membrane associated guanylate kinase, WW and PDZ domain containing 2 (Magi2) cDNA fragment under control of an 8.5 kb calcium/calmodulin-dependent protein kinase II alpha (CaMKIIα) promoter. The construct was microinjected into C57BL/6J fertilized oocytes, and founder mice were bred to C57BL/6J mice. S-SCAM transgenic founders were identified and were bred to C57BL/6J mice for at least 9 generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Expressed Gene | Magi2, membrane associated guanylate kinase, WW and PDZ domain containing 2, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Sang Lee |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | S-SCAM Tg |
Gene Symbol and Name | Tg(Camk2a-Magi2)1Shlee, transgene insertion 1, Sang Lee |
Gene Synonym(s) | |
Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory |
Expressed Gene | Magi2, membrane associated guanylate kinase, WW and PDZ domain containing 2, mouse, laboratory |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | The transgenic vector includes a 3.9 kb myc-tagged Magi2 (membrane associated guanylate kinase, WW and PDZ domain containing 2) cDNA fragment under control of an 8.5 kb calcium/calmodulin-dependent protein kinase II alpha promoter. |
When maintaining a live colony, transgenic mice may be bred to wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). The donating investigator reports that transgenic carriers are poor breeders and they have not attempted to make the colony homozygous.
When using the S-SCAM mouse strain in a publication, please cite the originating article(s) and include JAX stock #027306 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Camk2a-Magi2)1Shlee |
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